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Chapter 1-3

Chapter 1-3. Molecular Luminescence Spectroscopy. http://www.youtube.com/watch?v=YvN8zFhWn04. http://www.youtube.com/watch?v=Wf8NFRtJsHY&playnext=1&list=PLBE9D4F01340249F2. TIRFM. Total internal reflection fluorescence microscope. http://www.youtube.com/watch?v=Coe4T3Eky1k&NR=1.

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Chapter 1-3

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  1. Chapter 1-3 Molecular Luminescence Spectroscopy

  2. http://www.youtube.com/watch?v=YvN8zFhWn04 http://www.youtube.com/watch?v=Wf8NFRtJsHY&playnext=1&list=PLBE9D4F01340249F2

  3. TIRFM Total internal reflection fluorescence microscope http://www.youtube.com/watch?v=Coe4T3Eky1k&NR=1 http://www.youtube.com/watch?v=ONgz3RYZAeU&feature=related http://www.youtube.com/watch?v=YVBZMYifZoY

  4. (3) Energy level diagram: Deactivation of an excited state molecule 10-12 s 10-15 s 10-8 s 10-3 s forbidden QCA7e Chapter18

  5. Quantum Yields

  6. Quantum Yield

  7. Fluorescence lifetime ~ 10 ns

  8. Fluorescence quenching

  9. Q: iodide, oxygen, acrylamide

  10. Fluorescence anisotropy

  11. Fluorescence Anisotropy • Sample excited with linearly polarized light • Anisotropy, , of the resulting fluorescence measured • Reorientation of the molecule within the lifetime of the fluorophore will lead to a reduction in anisotropy

  12. Fluorescence energy transfer

  13. DNA hybridization

  14. Gene expression

  15. Steady-state and time-resolved fluorescence

  16. Fluorescence lifetime ~ 10 ns

  17. New fluorescence technologies • Multiphoton excitation • Fluorescence correlation spectroscopy • Single-molecule detection

  18. Multiphoton excitation

  19. Fluorescence Microscope inverted upright

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