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PCR Analysis of STK11 Gene Deletion for Identification of Genomic Changes

This study focuses on the XL-PCR analysis of two samples with a genomic deletion of exons 2-3 of the STK11 gene. The amplification results using specific primers in intron regions show a successful PCR in deletion carriers (patients 4688 and 4689) and not in a non-carrier sample. The Expand Long Template PCR system was utilized under optimized cycling conditions. The detection of a ~1400 bp deletion-specific band in carriers stands out, enabling the determination of the deletion breakpoint through direct sequencing.

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PCR Analysis of STK11 Gene Deletion for Identification of Genomic Changes

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  1. 10 kb Del+ Del+ Del- MW 10 kb 5 kb 2 kb 1.5 kb 1 kb 0.75 kb 0.50 kb 0.25 kb Figure S1: XL-PCR analysis of two samples carrying the genomic deletion of exons 2-3 of the STK11 gene The results of the PCR amplification using primers(marked by red arrowheads on the schematic drawing of the gene) in intron 1 (5'-CTGCCCTTTCCTGTCACTTC-3') and intron 3 (5'-AGCAGGCCCAGCAAAGAT-3') in deletion carriers (Del+; patients 4688 and 4689) and in a non-carrier (Del-) sample. The Expand Long Template PCR system (Roche) with Buffer 3 was used for amplification with the cycling conditions 93°C for 3 min, followed by 28 cycles of 93°C for 10 sec, 61°C for 30s and 68°C for 8 min and completed with a final extension step of 68°C for 20 min. Although solely a 8332 bp band was expected in the normal control sample, amplification was successful only in the deletion carriers, where a ~1400 bp deletion-specific band appeared which was then used for determination of the breakpoint by direct sequencing.

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