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A. A. 1 2 3 4 5 6. 1 2 3 4 5 6. 1 2 3 4 5 6. Control. Control. Control. 3+. 3+. 3+. Gd. Gd. Gd. B. B. C. C. V. faba. V. faba. V. faba. N. N.
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A A 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 Control Control Control 3+ 3+ 3+ Gd Gd Gd B B C C V. faba V. faba V. faba N. N. N. tobacum tobacum tobacum 3+ 3+ 3+ Control Gd Control Gd Control Gd 3+ 3+ 3+ Control Gd Control Gd Control Gd Pss Pss Pss + + + Pst avrRpm1 Pst avrRpm1 Pst avrRpm1 + + + Pst avrRpt2 Pst avrRpt2 Pst avrRpt2 3+ 3+ Supplemental Figure 1. Supplemental Figure 1. Gd Gd effects on growth of effects on growth of cultured on solid medium, and on pathogen cultured on solid medium, and on pathogen - - related related P. P. syringae syringae necrosis in necrosis in and and . . A A V. V. faba faba N. N. tabaccum tabaccum ( ( ) Overnight liquid LB cultures of ) Overnight liquid LB cultures of Pss Pss were washed and were washed and resuspended resuspended in 10 in 10 mM mM . . 8 8 MgCl MgCl 6H 6H O at 2 X 10 O at 2 X 10 cfu/mL cfu/mL and transferred to solid LB plates (‘Control’, upper panels) or and transferred to solid LB plates (‘Control’, upper panels) or solid LB medium containing 100 solid LB medium containing 100 2 2 2 2 3+ 3+ µM Gd µM Gd (lower panels). Undiluted culture is shown in lane 1, and seria (lower panels). Undiluted culture is shown in lane 1, and seria l 10 X dilutions of the culture are shown in lanes 2 l 10 X dilutions of the culture are shown in lanes 2 B B + + 8 8 through 6. ( through 6. ( ) ) N. N. tabaccum tabaccum leaves were inoculated with leaves were inoculated with Pst Pst avrRpm1 avrRpm1 (at 2 X 10 (at 2 X 10 cfu/mL cfu/mL ) with, or without (‘Control’) 100 µM ) with, or without (‘Control’) 100 µM + + 3+ 3+ C C Gd Gd ; pictures were taken after 48 h. ( ; pictures were taken after 48 h. ( ) ) V. V. faba faba leaves were inoculated with leaves were inoculated with Pss Pss (top panels) or (top panels) or Pst Pst avrRpt2 avrRpt2 (bottom panels) (bottom panels) 7 7 3+ 3+ (both pathogens at 1 X 10 (both pathogens at 1 X 10 cfu/mL cfu/mL ) without (left panels; ‘Control’) or with (right panels) 100 µM ) without (left panels; ‘Control’) or with (right panels) 100 µM Gd Gd ; pictures were taken after ; pictures were taken after 24 h. 24 h.