1 / 5

Characterizing Novel Aquatic Phages from the Environment

This study aims to characterize novel aquatic phages from the environment by isolating and characterizing their proteins and DNA. Techniques such as PCR, sequencing, and SDS-PAGE will be used to identify hosts that can be infected and determine protein characteristics. Interesting results from the gel electrophoresis will be verbally described.

coxs
Télécharger la présentation

Characterizing Novel Aquatic Phages from the Environment

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Characterizing Novel Aquatic Phages from the Environment 7.391 Minyoung Jang January 19, 2007

  2. Overview • Take water sample from the environment • Isolate: column, extract protein (CsCl), DNA (phenol chloroform) • Characterize: titer, identify hosts that can be infected, PCR, sequencing, digests

  3. SDS-PAGE • Denature isolated, purified proteins by SDS + boiling • Acrylamide gel • Load known samples & molecular weight (MW) markers

  4. Gel image here– LARGE, easy to see Label all lanes Label MW markers Label important bands Describe conditions: time run, voltage, & anything non-standard, how much you loaded ALL LABELS SHOULD BE EASY TO SEE! Describe interesting results VERBALLY (not written) How many different distinct bands do you see? Any particularly strong? What weight? Do any bands overlap with your “host cell” sample bands (ie those bands are not from phage) Etc. Be ready to answer questions about your protocol & data Our results

  5. Bioinformatics Homework • Protein identified • Source organism • Protein function • Amino Acid length • Known structures, family of proteins • Other functions of homologs • LIST ALL SOURCES!! (can footnote it & abbreviate [1] [1] Weigele, et al., 2007. Journ. Name Abbrev. Form.

More Related