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This study investigates the use of triplex-forming oligonucleotides (TFOs) in inducing specific mutations in mice. It addresses critical challenges such as cell uptake, TFO stability, specific targeting of sites, and the development of new DNA damage agents. The findings demonstrate that small DNA molecules can be effectively administered via intraperitoneal or intravenous injections, gaining access to the nuclei of tissue cells. This research supports the potential of nucleic acid aptamers as a more specific alternative to antibodies in medical and laboratory applications, including drug design and cell bioactivity regulation.
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Specific Mutation Induced by Triplex-Forming Oligouncleotides in Mice Karen M. Vasquez, Latha Narayanan, Peter M. Glazer 20 October 2000Science Date: 2001 4/25 Speaker: 劉育男
Introduction: ~Aptamer ~SELEX
TFO發展面臨的問題 1.Cell uptake 2.Stability of TFO 3.Specific targeting site or potential site 4.New DNA damage agent 5.Triplex-binding code
Conclusion: These studies established that small DNA molecule can be administered by i.p. or intravenous injection and gain access to tissue and to cell nuclei Chromosomal DNA throughout the somatic tissues of an animal can be targeted by nucleic acid aptamers
Aptamers的應用 ~取代antibodies 作為更專一性的結合ligand Medical usage, Lab usage ~調控Cell bioactivities Drug design
The End Thank you
