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Fast Protein Liquid Chromatography (FPLC )

Fast Protein Liquid Chromatography (FPLC ). Column components. 1. Packing. Gel degassing. Stopping the outlet of the column. Filling liquid in the bottom of the column. Pouring gell slurry to the column. Settling of gels. Adjusting the adaptor. Fixed adaptor. Conneting a line

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Fast Protein Liquid Chromatography (FPLC )

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  1. Fast Protein Liquid Chromatography (FPLC)

  2. Column components 1. Packing

  3. Gel degassing.

  4. Stopping the outlet of the column.

  5. Filling liquid in the bottom of the column

  6. Pouring gell slurry to the column.

  7. Settling of gels.

  8. Adjusting the adaptor

  9. Fixed adaptor

  10. Conneting a line to the adaptor

  11. Adaptor touching the upper layer of gels.

  12. Gel level lowers due to the solution flow into the column.

  13. Stopping the outlet line

  14. Connetion of pump line to the adaptor

  15. Blue dextran solution flows in the column.

  16. 2. Valve control

  17. Injection of sample

  18. 3. Remote controller FPLC controller.

  19. Pump front panel.

  20. Pump flow rate calibration

  21. Manual mode of gradient setting

  22. Valve number setting

  23. Flow rate setting

  24. 4. Multimeter Multimeter for digital data aquisition

  25. 5. Detector UV detector

  26. 6. Software(PROTEK 506) Software UV signal data aquisition

  27. Multimeter setting for sending data to notebook computer

  28. Sampling time setup

  29. File name.

  30. Save data file In a folder

  31. Starting data acquisition End ing data collection

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