Outside the Nucleus
In this laboratory exercise, students will measure the low power field of view (FOV) of their microscope and calculate the medium power FOV based on their findings. Specifically, a low power FOV of 6.0 mm leads to a medium power FOV of 2.4 mm. Students will also explore how many animal cells fit within this view and determine the size of each cell at 120 µm. Additionally, the lab emphasizes the essential role of ribosomes in protein synthesis, detailing the process from transcription in the nucleus to protein assembly in the rough endoplasmic reticulum.
Outside the Nucleus
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Presentation Transcript
Outside the Nucleus Do Now: Use your lab handout to answer. A student measures the low power field of view of their microscope to be 6.0 mm. A: What is the medium power F.O.V.? B: if about 20 animal cells fit in the medium power field of view, how big is each cell?
Medium power FOV • Medium FOV = (low power diameter)(low power objective) / (medium power objective) • = (6.0 mm)(4) / (10) = 2.4 mm
How big are the cells? • (Medium FOV) / (# cells) x 1000 • = 2.4 mm / 20 x 1,000 µm / mm = 120 µm
Label the cell membrane (aka plasma membrane), nucleus, and nucleolus on your diagram
About those ribosomes… • Very important function: RIBOSOMES MAKE PROTEINS! They can be found in both eukaryotic & prokaryotic cells
Steps in Protein Production • An RNA copy of DNA is made in the nucleus • A type of RNA called mRNA moves out of the nucleus into the rough endoplasmic reticulum (RER) • Ribosomes attached to the rough ER use the mRNA instructions to build a protein • The protein moves to the smooth ER for further processing. The smooth ER houses other chemical reactions too.
Homework: • CELL OBSERVATION LABS DUE TOMORROW!
