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G-Fectin TM

G-Fectin TM. 1. A novel transfection reagent developed by Genolution 2. Cationic lipid based 3. no cell splitting is required 4. Whole transfection can be done in 10 min 5. Your gene silencing in 24 hours 6. Lower toxicity than other reagents Better gene silencing

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G-Fectin TM

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  1. G-FectinTM • 1. A novel transfection reagent developed by Genolution • 2. Cationic lipid based • 3. no cell splitting is required • 4. Whole transfection can be done in 10 min • 5. Your gene silencing in 24 hours • 6. Lower toxicity than other reagents • Better gene silencing • For 500X transfection to one well of 24 well plate

  2. G-Fectin has good Transfection efficacy Transfection assay using Vimentin siRNA final 10 nM

  3. G-Fectin has low cytotoxicity Cell viability % All described cells were spitted in 30 % confluency in 24h ahead of viable cell counting with treatment of lipo-plexed RNA.

  4. Genolution siRNA 50 ul of PBS 1-ul ofG-factin Add the reagent & siRNA to the PBS 10 min transfection protocol with G-Fectin Incubate 10 min at RT Add to 24 well plate directly Cells seed on plate 450 ul of Suspended cells (5 x 104 cells/ well) Incubate 24 hrs qPCR

  5. siRNA Transfection assay kit G-Fectin KitTM The easiest and fastest way for siRNA transfection assaytest Just add to the cell, wait for 48hours, and count the viable cell The target gene of the used siRNA is essential for cell viability The cell toxicity and level of the gene silencing is proportional Materials included scramble siRNA control siRNA (cause to cell death if transfected, for 200X assay ) siRNA dissolving buffer G-factin 0.5ml (for 500 X transfection to 24 well)

  6. Easy siRNA Transfection assay kit Cell viability in 48 hour Scramble siRNA target siRNA Gene silencing in 24 hour

  7. Materials included scramble siRNA control siRNA (cause to cell death if transfected ) siRNA dissolving buffer G-factin 0.5ml (for 500 X transfection to 24 well)

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