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ANA 812

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ANA 812

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  1. ANA 812 Lab Session 11 Peripheral Blood Bryan Guillory Lashaundra Thedford Philip Overall John Watson

  2. Slide #42: Human Blood (Wright-Giemsa)Normal Blood (100X)

  3. Slide #42: Human Blood (Wright-Giemsa)Platelets (Thrombocytes) (1000x) Platelets ● Small (2-4 μm) ● Granular

  4. Slide #42: Human Blood (Wright-Giemsa)Erythrocytes (RBC’s)Normal Blood (400X) Erythrocytes • BiconcaveDiscs • -CentralPallor

  5. Slide #42: Human Blood (Wright-Giemsa)Normal Blood Erythrocytes (1000X) • Biconcave Discs • Central Pallor • 7-8 um in diameter

  6. Slide #43: Abnormal Human Blood (Wright-Giemsa)Anemic Blood (100X) • Smaller RBC’s • Larger Central Pallors • Variable Size and Shape

  7. Slide #43: Abnormal Human Blood (Wright-Giemsa)Anemic Blood (400X) • Smaller RBC’s • Larger Central Pallors • Variable Size and Shape

  8. Slide #43: Abnormal Human Blood (Wright-Giemsa)Anemic Blood (1000X) • Various Sized RBC’s • Larger Central Pallor • Variable Size and Shape

  9. Slide #42: Human Blood (Wright-Giemsa)Granulocytes: Neutrophils (400X) • Neutrophils • Granular Appearance

  10. Slide #42: Human Blood (Wright-Giemsa)Banded Neutrophils (400X) • “Stab Forms” • Nucleus Elongated • Less Prominent than Segmented Forms

  11. Slide #43: Abnormal Human Blood (Wright-Giemsa)Anemic Blood (1000X)Segmented Neutrophil • PMNs • Lobed Nucleus w/a Fine Filament Between • Most common Neutrophil

  12. Slide #42: Human Blood (Wright-Giemsa)Agranulocytes: Lymphocytes (1000x) Lymphocyte ● Round Nucleus ● Cytoplasm (blue halo)

  13. Slide #42: Human Blood (Wright-Giemsa)Agranulocytes: Monocytes (1000x) Monocyte ● Horseshoe, various shaped nucleus ● Large (14-20 μm)

  14. Slide #42: Human Blood (Wright-Giemsa)Granulocytes: Eosinophil (1000x) Eosinophil Bi-lobed nucleus Granules

  15. Electron MicrographGranulocytes: Eosinophil Lysosomal crystals Granule Nucleus

  16. Slide #42: Human Blood (Wright-Giemsa)Granulocytes: Basophil (1000x) Basophil ● “baso” because of blue-black granules when stained ● large granules that overlie nucleus (histamine) ● nucleus has smudged, indistinct chromatin pattern

  17. Slide #41: Peripheral Blood Film – Human (Wright-Giemsa)400x • Monocyte