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Natural Variation and the Genetic Basis of Altitudinal Adaptation in Arabidopsis thaliana

Natural Variation and the Genetic Basis of Altitudinal Adaptation in Arabidopsis thaliana. Yonghai Luo. ETH Zurich, Plant Ecological Genetics, Institute of Integrative Biology (IBZ) Universitätstrasse 16, 8092 Zurich, Switzerland. Global climate change and local adaptation.

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Natural Variation and the Genetic Basis of Altitudinal Adaptation in Arabidopsis thaliana

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  1. Natural Variation and the Genetic Basis of Altitudinal Adaptation in Arabidopsis thaliana Yonghai Luo ETH Zurich, Plant Ecological Genetics, Institute of Integrative Biology (IBZ) Universitätstrasse 16, 8092 Zurich, Switzerland

  2. Global climate change and local adaptation

  3. Light (Quality, Intensity, Duration, etc.) Temperature Moisture Atmosphere (CO2, O2, etc) Nutrition ... Factors affecting plant growth Internal Factors /Genetic Factors External Factors /Environmental Factors It is significant to uncover the interactions between genetic and environmental factors in order to understand the genetic basis of adaptation.

  4. Why Arabidopsis thaliana? • Little is known about the genetic basis of altitudinal adaptation in plants to date • Much is known about plant molecular biology from studies on A. thaliana • A. thalianaPopulations are found from high-altitude locations

  5. Project Goals: 1. To characterize genetic and phenotypic differentiation between high- altitude and low-altitude populations of Arabidopsis thaliana 2. To investigate the genetic basis underlying altitudinal adaptation.

  6. Experimental Approaches: Neutral loci vs phenotypic divergence Genome-wide sequence/expression variation Natural variation in candidate genes and pathways Manipulative environmental experiments Forward genetic experiments Genetics/ Genomics/ Mol. Biol.

  7. 16 populations from various sites and altitudes were collected in the Swiss Alps

  8. Genotyping of presumably neutral SSR loci

  9. F-statistics calculation -------------------------------------------------------- Locus Fwc(is) Fwc(st) Fwc(it) ------------ --------- --------- --------- M01 0.5590 0.6685 0.8538 M02 0.9331 0.8437 0.9895 M03 0.9998 0.8158 1.0000 M04 0.9861 0.6821 0.9956 M05 0.9864 0.6031 0.9946 M06 0.9999 0.7219 1.0000 M07 0.9688 0.6031 0.9876 M08 0.8667 0.7580 0.9678 M09 0.9472 0.6893 0.9836 M10 0.9633 0.7770 0.9918 ------------ --------- --------- --------- All: 0.9275 0.7211 0.9798 -------------------------------------------------------- Stenoien HK et al., 2005: 0.88 Le Corre V, 2005: 0.595

  10. Next steps • To finish Fst analysis (including neutral- and non-neutral loci) • To carry out Qst estimation • To identify loci for further analysis

  11. Acknowledgements All colleagues in the group of Plant Ecological Genetics! Thanks to all of you!

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