1 / 11

MicroRNA regulation in Arabidopsis thaliana

MicroRNA regulation in Arabidopsis thaliana. Diane Guo CUHK 6th IncoB, Aug.29, 2007. Outline. Background Characterization of miRNA targets Interactions between miRNAs and various cellular pathways Coordinated regulation by miRNAs and transcription factors Summary. microRNA.

iola
Télécharger la présentation

MicroRNA regulation in Arabidopsis thaliana

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MicroRNA regulation in Arabidopsis thaliana Diane Guo CUHK 6th IncoB, Aug.29, 2007

  2. Outline • Background • Characterization of miRNA targets • Interactions between miRNAs and various cellular pathways • Coordinated regulation by miRNAs and transcription factors • Summary

  3. microRNA • miRNAs are small endogenous RNA molecules (~22nt) found in plant and animals • Regulate gene expression through targeted mRNA cleavage or translational repression • Play critical roles in a variety of cellular processes such as development, cell proliferation, apoptosis, and stress response, etc

  4. MiRNA processing and activity (borrowed from Ambion)

  5. Mode of miRNA action in plants and animals (borrowed from Ambion)

  6. Table 1 GO terms of miRNA targets (molecular function)

  7. Table 2 Feature of miRNA target

  8. Table 2 Feature of miRNA target

  9. MiRNAs more frequently target genes with less transcription factor binding site

  10. Summary • We systematically analyzed the characteristics of miRNA targets. MiRNAs with multi-gene families are more likely to target TFs, and miRNAs represented by single gene tend to target non-TFs. • To systematically investigate the interactions between miRNAs and various cellular pathways, we analyzed the miRNA targets distribution in the pathways and found that miRNAs regulate the pathway through selectively interacting with positive regulatory modules and highly connected nodes. • We found that miRNA preferentially target genes bearing less TFBSs. This suggests that the post-transcriptional regulation by miRNAs is coordinated with regulation at the transcriptional level by transcription factors.

  11. Acknowledgement CUHK: Zhang Qing NEAU: Li Yong Zhu Yanming

More Related