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Association Mapping as a Breeding Strategy

Association Mapping as a Breeding Strategy. Mark E. Sorrells and Flavio Breseghello Department of Plant Breeding & Genetics Cornell University. Presentation Overview. A Genetic Model for Association Mapping in Plant Breeding Populations

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Association Mapping as a Breeding Strategy

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  1. Association Mapping as a Breeding Strategy Mark E. Sorrells and Flavio Breseghello Department of Plant Breeding & Genetics Cornell University

  2. Presentation Overview A Genetic Model for Association Mapping in Plant Breeding Populations Comparison of Different Plant Breeding Materials for Association Mapping Association Mapping of Kernel Size and Milling Quality in Soft Winter Wheat Cultivars

  3. Gene Marker Breeding Pool Gene={a} Marker={m,M} A Genetic Model for AM in Plant Breeding Populations:Association as Conditional Probabilities Population genetics theory (Hedrick 2005) Recombination (c) Recombination (c) Selection on A or M (w) New Parent (A,M) t generations Pr(A,M)=φ Pr(a,M)=θ Pr(a,m)=1-φ-θ Pr(A,m)=0 Pr(A|M,c,t,φ,θ,w) “Probability of a plant with marker allele M to have gene allele A, t generations after the introduction of A”

  4. θ=0 θ=0.05 θ=0.25 ~8 ~18 Recombination x initial frequency of M in the breeding pool Freq. new parent: φ=0.05 Relative fitness: w=1 Freq. M in original population = θ Freq. Recombination c θ=0 Pr(A|M) A novel marker allele at 10 cM distance can be more predictive of the QTL allele than an allele 1 cM away if it was present in the original pop at a freq of 0.05 t Generations

  5. Recombination x selection for M Freq. new parent: φ=0.05 Relative fitness: w = 4 (red), 2 (green),1.25 (blue) Freq. M in original pop: 0 Freq. Recombination: c = 0.01, 0.05, 0.10 • The generation at which the marker is depleted [Pr(A|M)=Pr(A)], depends on the selection intensity applied; • The final frequency of A depends on selection and tightness of linkage between marker and gene. Pr(A|M) Pr(A) Generations

  6. Summary • In plant breeding populations, the locus most associated with the trait is not necessarily the closest locus; • Loosely linked markers can still be useful for MAS if high intensity of selection is applied.

  7. MAS for Complex Traits: Issues • Accurate detection and estimation of QTL effects • Pre-existing marker alleles in a breeding population can be linked to non-target QTL alleles • Multiple QTL alleles can have different relative values • Gene x gene and gene by environment interactions

  8. Association Analysis as a Breeding Strategy • Most association studies have focused on estimating linkage disequilibrium and fine mapping. • Breeding programs are dynamic, complex genetic entities that require frequent evaluation of marker / phenotype relationships. Breseghello, F., and M.E. Sorrells. 2006. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 172:1165-1177. Breseghello, F., and M.E. Sorrells. 2006. Association analysis as a strategy for improvement of quantitative traits in plants. Crop Sci. In press.

  9. Association Mapping versus QTL Mapping • Association Mapping can be conducted directly on the breeding material, therefore: • Direct inference from data analysis to breeding is possible • Phenotypic variation is observed for most traits of interest • Marker polymorphism is higher than in biparental populations • Routine variety trial evaluations provide phenotypic data • Association Mapping provides other useful information about: • Organization of genetic variation in relevant breeding populations • Novel alleles can be identified and their relative value can be assessed as often as necessary

  10. Association Mapping versus QTL Mapping • Type I error (false positives) can be higher because of: • Unaccounted population structure • Simultaneous selection of combinations of alleles at different loci • High sampling variance of rare alleles • Type II error can be higher (low power) because of: • Lower LD than in biparental mapping populations • Unbalanced design due to differences in allele frequencies • A larger multiple-testing problem because of lower LD

  11. Integration of Association Analysis in a Breeding Program Germplasm Parental Selection Hybridization Elite germplasm feeds back into hybridization nursery New Populations Marker Assisted Selection Selection (Intermating) Novel & Validated QTL/Marker Associations New Synthetics, Lines, Varieties Evaluation Trials Elite Synthetics, Lines, Varieties Genotypic & Phenotypic data

  12. Types of Populations • Germplasm Bank Collection • A collection of genetic resources including landraces, exotic material and wild relatives. • Synthetic Populations • Outcrossing populations (either male-sterile or manually crossed) synthesized from inbred lines. May be used for recurrent selection. • Elite Lines • Inbred lines (and checks) manipulated with the objective of releasing new varieties in the short term.

  13. Characteristics Related to Association Mapping:Practical aspects

  14. Characteristics Related to Association Mapping: Genetic Expectations

  15. Characteristics Related to Association Mapping: Potential Applications

  16. Previous QTL information Width 2D • Doubled-Haploid Population AC Reed x Grandin • QTL for kernel size (width) near Xwmc18-2D • Recombinant Inbred Population Synthetic W7984 x Opata (ITMI population) • QTL for kernel size (length) on 5A and 5B Length 5B

  17. Association Analysis Materials • 95/149 soft winter wheat cultivars from the Northeastern US: Mostly recent releases, representing 35 seed companies / institutions • 93 SSR loci: 33 on 2D, 20 on 5A, 9 on 5B, 31 on 16 other chromosomes • Rare alleles (freq<5%):considered as missing for LD and population structure analysis; considered as allele for AM analysis Methods • Population Structure: 36 “unlinked” SSR markers- Structure without admixture, SPAGeDi (Hardy & Vekemans) program for Kinship ; Visualization: Factorial (Multiple) Correspondence Analysis (Benzecri, 1973 L' Analyse des correspondances. Dunod) • Linkage Disequilibrium:Tassel (maizegenetics.net) used to compute r2 , with p-values from 1000 permutations • Association Analysis:R stats package lme used to analyze Linear mixed-effects model with marker as fixed effects (selected from previously identified QTL regions) and subpopulations or Kinship as random effects (no obvious differentiating characteristics); Two-marker models: tested by likelihood ratio test • Jianming Yu, Gael Pressoir, et al. (2006) A Unified Mixed-Model Method for Association Mapping Accounting for Multiple Levels of Relatedness Nature Genetics 38:203-208

  18. Estimating Relatedness The K Matrix j . . . . Fij= (Qij-Qm)/(1-Qm) (Ritland, Loiselle) If Fij is negative, then it is set to zero. F11 . . . . Θij≅Fij …………. i Fnj…… Fnn Relatedness (K) In cattle studies the analogous matrix is estimated from pedigrees, and it controls for the polygene effect Jianming Yu, Gael Pressoir, et al. (2006) A Unified Mixed-Model Method for Association Mapping Accounting for Multiple Levels of Relatedness Nature Genetics 38:203-208

  19. Population Structure:Sample Subdivisions • Subpopulation No. of Varieties Fst • 19 0.337 • 32 0.111 • 13 0.295 • 31 0.064 Total 95 0.188 Moderate Population Subdivision

  20. Population Structure:Factorial Correspondence Analysis Orthogonal views of 4 soft winter wheat subpopulations S2 S3 S4 S1

  21. Linkage Disequilibrium:Germplasm Sample Selection R2 probability for unlinked SSR markers • 149 lines genotyped with 18 unlinked SSR markers • Most similar lines were excluded • "Normalizing" the sample drastically reduced LD among unlinked markers 149 lines 95 lines

  22. Normal curve LD baseline LD baseline Normal Distr. 95th percentile Definition of a baseline-LD specific for our sample Defined as the 95th percentile of the distribution of r2 among unlinked loci r2 estimates above this value are probably due to genetic linkage Baseline LD for this sample: r2 = 0.0654

  23. Linkage Disequilibrium: Chromosome 2D Consistent LD was below 1 cM, localized LD 1-5 cM

  24. ~5 cM Linkage Disequilibrium: Chromosome 5A Significant LD extended for 5 cM in pericentromeric region

  25. Likelihood Ratio Test Loci Associated with Kernel Size (p-values)Chromosome 2D Agreed with QTL in Reed x Grandin Kernel Size ** Milling Quality None of the loci on 2D were significant after multiple testing correction

  26. Likelihood Ratio Test n.s. ** Loci Associated with Kernel Size (p-values)Chromosome 5A Agreed with QTL in M6 x Opata Kernel Size Milling Quality

  27. B.L.U.E. of allele effects Kernel Length N. of Cultivars: 951837 9 9 41454349

  28. B.L.U.E. of allele effects Kernel Width N. of Cultivars: 4114 8 151824 5 10 19

  29. B.L.U.E of allele effects Kernel Weight N. of Cultivars:41454349

  30. Conclusions • Linkage Disequilibrium • Variation in LD across the genome can be characterized in relevant germplasm • Markers closely linked to QTL of interest can be identified and allelic effects quantified • Association Mapping as a Breeding Strategy • For recurrent selection, markers could be used to carry information from a “good year” to a “bad year” • In pedigree breeding, markers could carry information about traits of interest from replicated field trials to single row or single plant selection • Allelic values of previously identified alleles can be updated annually based on advanced trial data combined with genotypic data • New alleles can be identified and characterized to determine their relative value • A selection index can be used to incorporate both phenotypic and molecular data

  31. USDA Soft Wheat Quality Lab, Wooster, OH Embrapa Acknowledgements Technical Support: • David Benscher • James Tanaka • Gretchen Salm

  32. Kangaroo Island Wayne Powell

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