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Understanding the Electrocardiogram and Blood Typing Procedures in Circulatory Physiology

This guide outlines key procedures for recording heart electrical activity and blood typing. Learn about the P wave, QRS complex, and T wave which depict atrial and ventricular activity in the electrocardiogram. Additionally, discover methods to determine pulse rate and measure systemic blood pressure in various locations. The blood typing procedure includes using synthetic blood and anti-serums to identify blood types, focusing on agglutination reactions. Essential for students in physiology and medical studies, this resource provides practical insights into circulatory system assessments.

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Understanding the Electrocardiogram and Blood Typing Procedures in Circulatory Physiology

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  1. Laboratory Eleven The Circulatory System: Physiology

  2. The Electrocardiogram: Recording of Heart Electrical Activity(p202, 11A Procedure, #2) • P wave • Atrial depolarization • Occurs before atrial contraction • Generated by the firing of Sinoarteial (SA) node • QRS complex • Ventricular depolarization • Occurs before ventricular contraction (systole) • T wave • Ventricular repolarization • Occurs before ventricular relaxation (diastole)

  3. Pulse & Blood Pressure(p203, 11B Procedure) • Determine pulse rate manually in 5 different locations • Measure systemic blood pressure using the peripheral pulses in the brachial artery • Systolic pressure (first soft sound): generated by contraction of left ventricles • Diastolic pressure (sound terminates): corresponds to relaxation of the left ventricle

  4. Blood Typing

  5. Anti-B B Rh Anti-Rh A Anti-A Blood Typing(p205, 11C Procedure) • 2 drops of the synthetic blood of only one subject • 2 drops of each anti-serum • Mix with toothpicks (avoid cross contamination) • Place the slide on the Rh-typing box for 30-45sec while gently rocking the box • Observe the wells for agglutination (RBC clumping) • Record your results in your lab report • e.g.: • B- (agglutination in anti-B well) • O+ (agglutination in anti-Rh well) • AB+ (agglutination in Anti-A, -B,& -Rh wells)

  6. A Typical Antibody

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