Transcription factor genes

1. Col-0 MeJA 0.04 jin1-9 MeJA At3g28740/P450 0.035 Col-0 mock 0.03 jin1-9 mock 0.025 0.02 relative expression ERF1 ERF6 0.02 0.015 0.015 0.01 0.01 0.005 0 0.005 1h 3h 6h 24h 0 0.16 0.1 ERF8 ERF11 TDR1 0.03 0.08 0.12 0.06 0.02 0.06 0.08 Col-0 MeJA Col-0 MeJA At1g33760 0.18 0.12 0.04 0.05 jin1-9 MeJA Col-0 MeJA jin1-9 MeJA 0.16 3 0.01 AOC4 ACS6 0.04 Col-0 mock 0.1 Col-0 mock jin1-9 MeJA 0.02 0.04 0.14 AACT/At5g61160 jin1-9 mock 2.5 jin1-9 mock Col-0 mock 0.12 0.08 0.03 0 0 0 jin1-9 mock Relative expression relative expression 0.1 2 0.02 0.06 0.6 0.04 0.2 At1g06160 relative expression WRKY26 WRKY33 0.08 1.5 0.01 0.06 0.04 0.5 0.03 0.15 0.04 1 0 0.4 0.02 0.02 0.5 0.3 0.02 0.1 0 0 1h 3h 6h 24h 1h 3h 6h 24h 0.2 0 0.01 0.05 1h 3h 6h 24h 0.1 0 0 0 0.25 At5g04340 ZAT10 Transcription factor genes 0.3 0.2 0.15 0.2 0.1 0.1 0.05 1.2 0.6 ERF2 ERF4 1 0.5 0 0 0.8 0.4 0.3 MYB75/PAP1 ERF13 0.006 0.6 0.3 0.25 0.4 0.2 0.2 0.004 0.2 0.1 0.15 0 0 0.1 0.002 0.05 0 0 1h 3h 6h 24h 1h 3h 6h 24h Relative expression 1h 3h 6h 24h Col-0 MeJA jin1-9 MeJA Col-0 mock jin1-9 mock Phenylpropanoid pathway genes Phytohormone biosynthesis genes 1h 3h 6h 24h Supplemental Figure 1: Q-RT-PCR expression analysis of selected MYC2-regulated genes in jin1-9vs. Col-0, after MeJA or mock treatment. Q-RT-PCR expression analysis of genes after treatment with 0.1 mM MeJA (black symbols) or mock (white symbols) treatment in Col-0 (squares) and jin1-9 (triangles). Data are expressed as relative RNA levels ([mRNA]gene/[mRNA]actin) and are means of three biological replicates (>30 pooled plants each); error bars denote standard errors. See Methods for further information and Supplemental Table 3 for primer sequences used in the Q-RT-PCR experiments.