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Microfluidic Droplets for DNA Sequencing and Materials Synthesis

Microfluidic Droplets for DNA Sequencing and Materials Synthesis. Juila E. Fulghum , University of New Mexico, DMR 0611616.

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Microfluidic Droplets for DNA Sequencing and Materials Synthesis

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  1. Microfluidic Droplets forDNA Sequencing and Materials Synthesis Juila E. Fulghum, University of New Mexico, DMR 0611616 We demonstrated successful detection of single exon in RNA fragments locked in microdroplets. The figure below shows a collection of droplets that are loaded with different RND fragments and primers to amplify a 100 bp of specific exon (Exon 9.) The obtained results demonstrated that only the targeted exon 9 can be amplified and detected by a complementary molecular beacon that gives a fluorescent signal. As a control, beacons for Exons 10 and 9A were added to the solution, but they gave no response since they were not amplified. Hierarchically bimodal porous structures were obtained templating silica microparticles with a specially designed surfactant micelle/microemulsion mixture. We examined the dynamics of the formation of these biporous structures. In order to obtain a better understanding of the structural evolution and stability, we have performed surface tension and dynamic light scattering measurements of the surfactant/water/oil systems. These experiments provided valuable insights about the time changes of the governing parameters (surface tension, surface charge, background electrolyte concentration) and their effect on the phase state of the oil/water/surfactant system. The obtained data are correlated to the final porous structure of the silica particles. Bright Field Exon 9S Cy5 Exon 9A Cy3 Exon 10 FITC N. J. Carroll*, S. Pylypenko, A. Ortiz*, B. T. Yonemoto*, C. Lopez*, P. Atanassov, D. A. Weitz, and D. N. Petsev, Droplet Based Microfluidics for Synthesis of Mesoporous Silica Microspheres, MRS Symposium Proceedings, 1272 (2010) KK08-02. C. E. Ashley, E. C. Carnes, G. K. Phillips, D. Padilla, P. N. Durfee, P. A. Brown, T. N. Hanna, J. Liu, B. Comyford, M. B. Carter, N. Carroll*, X. Jiang, D. R. Dunphy, C. L. Willman, D. N. Petsev, D. G. Evans, A. Parikh, B. Chackerian, W. Wharton, D. S. Peabody, and C. J. Brinker, Targeted Delivery of Multicomponent Cargos to Cancer via Protocell-Like Nanoporous Particle-Supported Lipid Bilayers, Nature Materials, accepted. Funding for this research was provided by NSF PREM Grant # DMR-0611616.

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