1 / 23

Efficient 10 µL Transfer Protocol Between Plates Using Automated Pipetting

This protocol outlines a simple 6-line program to transfer 10 µL from a source plate to a destination plate using an automated pipetting system. It includes tips on washing, moving to aspirate and dispense positions, and effective dispensing techniques to ensure accurate transfer. The setup features a column of tips and stumps, ensuring efficient operation. The program highlights key steps such as filling tips, dispensing into rows, and ensuring drops settle at the bottoms of wells for successful processing.

duc
Télécharger la présentation

Efficient 10 µL Transfer Protocol Between Plates Using Automated Pipetting

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Transferring 10uL between plates

  2. A simple 6 line program

  3. Source plate

  4. Destination plate

  5. Tip washing station

  6. Tips washing

  7. Moving to the aspirate position

  8. The aspirate position at the source plate

  9. Aspirating

  10. Moving to the dispense position

  11. Dispensing

  12. Preparing to wash after transfer

  13. Washing

  14. Dispensing 1uL from a source row to all wells of another plate

  15. Head setup with one column of tips and one column of “stumps”

  16. Dispense head set up and installed on the Apricot

  17. Beginning to aspirate from source wells

  18. Filling tips with 25uL

  19. Dispensing first column

  20. Moving to next column

  21. Dispensing a later column

  22. The plate is done

  23. Note how all drops are at the bottoms of the wells

More Related