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RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT

RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT. J. Steigert, L. Riegger, M. Grumann, T. Brenner, J. Harter, R. Zengerle, and J. Ducr é e IMTEK – University of Freiburg, Laboratory for MEMS Applications Georges-Koehler-Allee 106, D-79110 Freiburg, Germany

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RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT

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  1. RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT J. Steigert, L. Riegger, M. Grumann, T. Brenner, J. Harter, R. Zengerle, and J. Ducrée IMTEK – University of Freiburg, Laboratory for MEMS Applications Georges-Koehler-Allee 106, D-79110 Freiburg, Germany (μTAS2005 P.1~3) Reporter: Chung-Kuang Chin(秦重光) Date:11/28/2007

  2. Outline • Introduction • Design & Theory • Results • Conclusion

  3. Introduction • routines in clinical diagnostics (resource consumption) －＞compact point-of-care devices －＞lab-on-a-chip (Bio-MEMS) • disk-based, colorimetric alcohol assay & optical beam-guidance integrated system to rapidly determine the concentration of alcohol in human whole blood Lab on a disk－＞

  4. Design & Theory (1) • Disk-based platform －various microfluidic functions －polymer (COC) disk (bio-compatible) －high-throughput screening (HTS) Ref: www.answers.com/topic/data-recovery

  5. Design & Theory (2) • Optical path setup －total internal reflection (TIR) －extend optical path ( increase absorption )

  6. Design & Theory (2) • Total internal reflection (TIR)

  7. Design & Theory (2) • extend optical path －law of Beer-Lambert

  8. Design & Theory (3) ☆ • ALCOHOL ASSAY Vs = 500 nl Vr = 99.5 μl C.V. (sample metering) = 1.7 %

  9. Design & Theory (3) ☆ • ALCOHOL ASSAY

  10. Results The performance is comparable to common breath analyzers

  11. Conclusion • This paper introduced a highly process integrated disk-based assay to rapidly and accurately determine the concentration of alcohol in human whole blood • The assay is run on a cost efficient modular platform comprising a passive polymer disk with monolithically embedded optical and fluidic structures as well as a standard laser diode and detector • Its high degree of automation, its short time-to-result and its excellent precision recommend our centrifugal platform for point-of-care testing and routine screening in clinical labs

  12. THE END Thanks for Your Attention!

  13. Reference • M. Grumann et al., Proc. of MEMS 2005 conf., pp. 108–112, (2005) • Jitae Kim,a Seh Hee Jang,b Guangyao Jia,a Jim V. Zoval,a Nancy A. Da Silvab and Marc J. Madou*a , Cell lysis on a microfluidic CD (compact disc) ,Lab on a chip , pp.516-522, (2004)

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