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Risk Assessment

Risk Assessment. Progress on the 10 Risks identified September 2012. Priorities. Nurseries National Structures Risk Management Variation to National Phylloxera Management Protocol Consistent language Protocol – PQS Review of NPMP. 5 year plan.

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Risk Assessment

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  1. Risk Assessment Progress on the 10 Risks identified September 2012

  2. Priorities • Nurseries • National Structures • Risk Management • Variation to National Phylloxera Management Protocol • Consistent language Protocol – PQS • Review of NPMP

  3. 5 year plan • PGIBSA has commenced a process of developing its next 5 year plan and invited WGCSA and SAWIA to participate. • The outcome is to develop a clear plan that major stakeholders have buy in • To better use our limited resources to meet the priorities of the SA grape growing industry

  4. PGIBSA current actions • Online Grape Industry Kiosk (vineyard register) • Online Risks • DNA research • Incidence reporting as a basis for education • Regional outbreak planning and risk assessment

  5. DNA Sampling project A collaborative project between PGIBSA, SARDI, University of Adelaide, Rho. Environmentrics, GWRDC and PBCRC

  6. Overview • WHY? • WHAT? • WHEN? • HOW? • RESULTS • NEXT STEPS

  7. WHY? • Need for a cost effective surveying tool which is easy to use and provides comfort to stakeholders • Need for a method of sampling that owners can carry out • Method is scientifically proven and stands the test of international scrutiny • DNA sampling to add value by providing additional information on pests and management.

  8. WHAT? • DNA testing can identify Phylloxera • What we don’t know is the size of sample needed • Where the sample needs to be taken from – position and depth • The sampling frequency for a 95% confidence level • The impacts on sampling in different soils/locations and times of the year • Determine the best tool for collecting samples

  9. WHEN? • Samples collected in March 2013 • Samples collected in Yarra Valley • There will be progress reports along the way in accordance with contractual arrangements

  10. HOW? - METHODOLOGY • Vines visually inspected to confirm Phylloxera is present • 2 dig sticks tested one large (>140gm), one small (<50gms) on average • 4 samples for each dig stick at dripper or trunk/vine • Depth of 0-20cm = 120 samples • All samples were refrigerated for transport to testing lab

  11. HOW? – METHODOLOGY for DNA STABILITY • 4 samples were taken from each side of a vine and randomised in to categories 2, 3, 5 and 12 days • Samples from each side of the vine were used to compare sampling tools • Vines within a replicate were randomised to storage at 10°C, 20°C and 35°C before DNA testing • There were 5 replicates

  12. RESULTS of STABILITY TRIAL • 116 of the 120 samples tested detected Phylloxera DNA • There was no significant difference between the size of dig stick used or the location e.g. trunk or dripper • The 4 samples that showed no Phylloxera DNA had been stored at 35°C for 12 days • Half lives at 10°C and 35°C were 3.5 and 2.3 days • Overall 97% detection rate of Phylloxera from these samples

  13. Where are we up to? • Have confirmed sample viability • Have completed sampling 4 during 2013 • DNA identified at the same level of significance for March, July and September data collections(December results yet to be analysed) even though the numbers of Phylloxera decreased.

  14. Next Steps • Test DNA on sites with no detected Phylloxera to test for false positives (Riverland, Barossa and Mundulla) • Test sampling tools in rootstocks sites in PIZ’s • Repeat the trial for 2014 and 2015 • 2016 promotion of outcomes

  15. Acknowledgements • Ray Correll - Rho.environmetrics • Alan McKay - SARDI • Kathy Ophel-Keller - SARDI • Cassandra Collins - University of Adelaide • Andrew Downs - PGIBSA • Danièle Giblot-Ducray - SARDI • Greg King - DEPI-Vic • Chris Anderson - DPI-NSW

  16. Questions Alan Nankivell alann@phylloxera.com.au Thank You

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