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RNA. R ibo N ucleic A cid. Also made of monomers called nucleotides 5 carbon sugar: Ribose Phosphate functional group: PO 4 1 of 4 nitrogen bases Cytosine Guanine Adenine Uracil replaces Thymine Molecule is a single strand. Purpose of RNA.
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RiboNucleic Acid Also made of monomers called nucleotides • 5 carbon sugar: Ribose • Phosphate functional group: PO4 • 1 of 4 nitrogen bases • Cytosine • Guanine • Adenine • Uracil replaces Thymine • Molecule is a single strand
Purpose of RNA There are many types of RNA, so function varies depending upon type: • Directly involved in converting genetic code on DNA into proteins • Determines which genes are expressed • Some regulate chemical processes, so, they function like enzymes • Small sections of RNA act as place holders for DNA polymerase during DNA replication
3 TYPES OF RNA USED IN PROTEIN SYNTHESIS messenger RNA(mRNA) • Long, single strand • Made in the nucleus • Carries genetic code from DNA to ribosomes In cytoplasm
transfer RNA (tRNA) • Folded chain of RNA • Carries the amino acid monomers that make a protein • “Translates” the genetic code
ribosomal RNA (rRNA) Ribosomes • Globular strand of RNA • 2 parts • Located on the ER and free floating in cytoplasm • Site of protein synthesis
Why is RNA needed in the first place? DNA carries the code, however, DNA cannot leave the nucleus. RNA carries the “genetic message” to ribosomes Why change a base? The nuclear envelope “recognizes” the bases on DNA and keeps it in the nucleus; by changing a single base on RNA, its chemical signature changes, so the nuclear envelope will allow it to pass through to cytoplasm Why not just make proteins in the nucleus? Proteins are “huge” molecules. There’s just not enough room in the nucleus to make all the proteins an organism requires, so the protein factory (ribosomes, ER and Golgi Body) is out in the cytoplasm where there’s plenty of space. Why are ribosomes on the ER and in the cytoplasm? Proteins are needed for many functions. The proteins made in ribosomes on the ER are transported either to the nucleus or out of the cell(hormones). Those ribosomes in the cytoplasm make proteins that stay in the cell(replace organelles)
MAKING mRNA1st step in protein synthesis Messenger RNA is made in the nucleus in a process called: TRANSCRIPTION Here the genetic code on DNA is “rewritten” in a strand of mRNA. BIRTHDAY CAKE
A B C ONCE THE RNA IS FORMED IT DETACHES FROM THE DNA TEMPLATE. THE DNA EITHER CLOSES OR TRANSCRIPTION CONTINUES UNTIL ENOUGH mRNA IS MADE FOR PROTEIN SYNTHESIS
Next Steps…. 2. The newly formed messenger RNA leaves the nucleus 3. Together with the ribosomes and tRNA a new protein is formed during TRANSLATION
TRANSLATION • THE ORDERING OF AMINO ACIDS BY mRNA TO FORM A POLYPEPTIDE(PROTEIN) • TRANSLATION TAKES PLACE IN RIBOSOMES. (ROUGH E.R. OR CYTOPLASM) • EACH TYPE OF RNA CONTROLS THE ORDER OF AMINO ACIDS Let’s see how the code is read…
MESSENGER RNA (mRNA) CODONS • The genetic code is translated in ‘3 letter words” • mRNA – THREE NUCLEOTIDE BASES MAKE A CODON. • A CODON CODES FOR A SPECIFIC AMINO ACID.
TRANSFER RNA – (tRNA)The Translator • THREE NUCLEOTIDES AT THE HEAD(one end) OF THE tRNA IS THE ANTICODON • THE ANTICODON WILL MATCH UP WITH THE mRNA CODON • THE TAIL OF THE tRNA CARRIES THE AMINO ACID THAT CORRESPONDS TO THE CODON.
THE RIBOSOME • CELL ORGANELLE THAT IS MADE UP OF rRNA. • SITE OF PROTEIN SYNTHESIS • RIBOSOME HAS 2 ‘PARKING PLACES’ FOR tRNA • A site: tRNAs enter • P site: Amino Acids bond • E site: tRNA exits(not shown)
SUMMARY OF TRANSLATION 1. The small ribosome subunit attaches to the mRNA at the start codon. • This START codon is always AUG! • Then the large subunit of the ribosome attaches over the small subunit #1
#2 2.The A site of the ribosome is filled with the first tRNA carrying the first amino acid (always Methionine) • The ribosome shifts down one codon, moving the tRNA to the P site and opening the A site again
#3 3. The mRNA codon is ‘read’ and the second corresponding tRNA carrying the second amino acid fills the A site 4. At this time apeptide bond is formed between the two amino acids in the A and P sites PEPTIDE BOND #4
5. The firsttRNA in the P site releases its amino acid and is moved to the E site where it is released from the ribosome 6-7.The ribosome again shifts down 1 codon, moving the second tRNA to the P site leaving the A site empty for the third tRNAcarrying the next amino acid 8. This process of building the polypeptide chain continues until the ribosome reaches the STOP codon at the end of the mRNA and detaches #5 #6-#8
mRNA Codon CAG Start with first letter in center and work your way out for each letter in the codon until you identify the amino acid GLUTAMINE READING THE AMINO ACID WHEEL 6 There are only 20 amino acids(that is 20 monomers) for all proteins. However, there are far more than 20 codons. For example: Find LEUCINE( be careful, its listed more than once…how many codons are there for this one amino acid? What might this imply about certain amino acids? Amino acids that are used more often in proteins have more than one codon; this helps increase the rate of protein synthesis and decreases chance mutation will alter the protein.
Let’s Practice! Write the DNA sequence below, in one color; TAC CTT AAC GAG CTA AAA GTT AGC TGG TTG ACT Now, transcribe the mRNA codons from the DNA; AUG GAA UUG CUC GAU UUU CAA UCG ACC AAC UGA Good Job! Now, what would the tRNA anticodon’s be? UAC CUU AAC GAG CUA AAA GUU AGC UGG UUG ACU Awesome! Now, let’s make a polypeptide by translating the mRNA codon’s to amino acids: MET- GLU A-LEU-LEU-ASP A-PHY-GLU-SER-THR-ASP-STOP