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A. B. ***. **. ***. ***. ***. ***.

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  1. A B *** ** *** *** *** *** For osteoclast-T cell co-culture, bone marrow cells (BMCs) were isolated from 4- to 6-week-old mice by flushing the bone marrow from the excised femora with osteoclast cell culture medium containing 10% FCS. After overnight culture in this medium, non-adherent bone marrow cells were added to purified CD4+CD28null T cells in 24-well plates in osteoclast culture medium containing M-CSF and RANKL and culture was continued till day 6. At the end of experiment conditioned media was collected to perform TNF-α ELISA (A). The transcript level of tartarate resistant acid phosphatase (TRAP) in co-culture was determined by q-PCR (B). ***P < 0.001

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