1. Genome Presentation Schizosaccharomyces Pombe Anita Kim BME 088a - Surfing your Genome Prof. Todd Lowe February 20, 2003

2. Background Related Species • Fission yeast • A single-celled fungus • Eukaryotic • Found in Millet beer from East Africa in 1893 • P. Linder • “Pombe” is the Swahili word for beer • 6th Eukaryote genome sequenced • Saccharomyces Cerevisiae • Sequenced after S. Pombe • 12 Mb • 13 Chromosomes Number of Articles Found From NCBI PubMed: 5,061

3. February 21, 2002 The Wellcome Trust Sainger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK Led by Dr. Paul Nurse Among the winners of the 2001 Nobel Prize for his research on the cell cycle in fission yeast When and Who?

4. The Sequencing • Found on 50 different genes • 13.8 Mb (Megabases • Has 3 chromosomes • Chromosome I: 5.7 Mb • Chromosome II: 4.6 Mb • Chromosome III: 3.5 Mb • Contains lowest number of protein coding genes recorded for a Eukaryote • 4,824 protein-coding genes • 33 pseudogenes • G+C (guanine and cytosine) content: 36.0%

5. Why was it sequenced? • Associated with human diseases such as cancer, which is found on 23 of the 50 genes • Cystic Fibrosis, hereditary deafness, and diabetes • Protein-coding genes identified that generate products with similarities to proteins coded by 289 genes that are mutated, amplified or deleted in human disease.

6. Bibliography • The Forsburg Lab at the Cancer Center in the Sulk Institute in La Jolla, California http://pingu.salk.edu/~forsburg/ • National Center for Biotechnology Information http://www.ncbi.nlm.nih.gov/ • http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11859360&dopt=Abstract • The Wellcome Trust Sanger Institute http://www.sanger.ac.uk/Projects/S_pombe/ • http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v415/n6874/full/nature724_r.html