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Toxicity of Automotive Runoff on Daphnia.

Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10 th Grade Bellwood-Antis. Why I Chose this Experiment. I chose this experiment because…. Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments.

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Toxicity of Automotive Runoff on Daphnia.

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  1. Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10th Grade Bellwood-Antis

  2. Why I Chose this Experiment I chose this experiment because… • Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments. • Earlier in the year I had learned a little about Daphnia.

  3. Background Information • Brake fluid • Contains: Diethylene glycol (C4O3H) Windshield Washer Fluid • Contains: Methanol (CHO3H) • Antifreeze • Contains: Ethylene glycol (C2H6O2) Lethal Dose 50 in 96 hours

  4. Hypothesis Windshield washer fluid be the least lethal.

  5. Why I Chose Daphnia… • In our experiment we used the variety of daphnia known as Daphnia Magnum. • Daphnia being much smaller, would be more susceptible. Because they are a food source, this could then work its way up the ecosystem. • Daphnia are an indicator species.

  6. General Procedure • 40ml fluid with 4 daphnia • 3 trials of each dilution

  7. Making a dilution: Ex. 1/10 dilution • Water: 34ml • Automotive pollutant: 4ml • Food:2ml

  8. Recipes For My Dilutions Dilution Water Food Chemical 1/2 18 2 20 1/5 30 2 8 1/10 34 2 4 1/20 36 2 2 1/40 37 2 1 1/80 37 ½ 2 ½

  9. Experimental Design • Variable • the pollutant • Experimental Group • Antifreeze, Brake Fluid, and Windshield Washer Fluid • Control Group • Spring Water

  10. Experimental Design Contd. • Control Variables: Amount of… • Light • Food • Chemical • Water Others… • Temperature • Size of daphnia • Clean materials

  11. LD 50 • Daphnia died too close together

  12. Daphnia Lifetime (in minutes) inBrake Fluid

  13. Averages Daphnia Lifetimes of 3 Trials Note: All numbers are in minutes.

  14. Conclusion According to my data, I reject my hypothesis that Windshield Washer Fluid would be the least lethal to daphnia.

  15. Improvements • I would… • use more specific dilutions. • use different animals. • use more chemicals.

  16. Expanding my Project • I could… • test motor oil by using a larger aquatic animal. • test to see what affect making the water more acidic or basic would have.

  17. Bibliography • http://ijt.sagepub.com/cgi/content/abstract/23/1/55 1/24 • http://www.aehms.org/jaeh_3_2_sherry.html 1/24 • http://www.jtbaker.com/msds/englishhtml/e5125.htm 2/8 • https://fscimage.fishersci.com/msds/89493.htm 2/8 • http://www.atsdr.cdc.gov/tfacts96.html 2/10

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