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Review: Fast Scanning Methods

Review: Fast Scanning Methods. Acoustic Optical modulator Many other uses too. Spinning Disk Confocal Low excitation throughput. Laser Trapping / Laser Cooling / Laser Tweezers. pN forces n > n(surroundings) The best setup “cools” particles motion down to Å range at ms timescale.

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Review: Fast Scanning Methods

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  1. Review: Fast Scanning Methods • Acoustic Optical modulator • Many other uses too. • Spinning Disk Confocal • Low excitation throughput

  2. Laser Trapping / Laser Cooling / Laser Tweezers • pN forces • n > n(surroundings) • The best setup “cools” particles motion down to Å range at ms timescale.

  3. Fluorescence Lifetime Imaging • Motivation: measures amount of “quenching” directly. • Frequency domain methods – uncommon • Time domain methods: • Time-gated detection with ICCD • Wide-field method – no scanning • Not photon efficient • Time-correlated single photon counting • Point-by-point scanning necessary • Most accurate

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