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Selected and Differential Media

Selected and Differential Media. Define . Selective Media Differential Media Enriched Media. Blood Agar Plate (BAP). Differential Enriched Use Growth Hemolysis Alpha Beta Gamma. C-CNA. Selective Differential Results Gram (+) Colistin Naldixic acid Blood Hemolysis. MSA.

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Selected and Differential Media

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  1. Selected and Differential Media

  2. Define • Selective Media • Differential Media • Enriched Media

  3. Blood Agar Plate (BAP) • Differential • Enriched • Use • Growth • Hemolysis • Alpha • Beta • Gamma

  4. C-CNA • Selective • Differential • Results • Gram (+) • Colistin • Naldixic acid • Blood • Hemolysis

  5. MSA • Selective • Differential • Results • Halotolerant (7.5% NaCl) • CH2O: Mannitol • pH • phenol red • Fermentation • Acid • Yellow

  6. MAC • Selective • Differential • Results • Gram (-) • CV • Bile Salts • Lactose • pH indicator • Neutral red indicator • Ferment = pink/red • No acid = colorless

  7. EMB • Selective • Differential • Results • Gram (-) • Eosin dye • MB dye • Lactose • Some acid = pink • Large acid = green • No acid = colorless

  8. Set Up • One plate for each group • Label quadrants as lab book indicates for each plate • Cultures on cart • Anaerobic Gas Pack • BAP • C-CNA • All others aerobic • 37°C

  9. BAP results • Sheep blood enriched • Growth most all microbes • Fastidious • Differential • GP Hemolysins • Hemolysis types • Gamma • Alpha • Beta

  10. Hemolysis No hemolysis

  11. C-CNA results • Selective • Growth GP • Colistin • Nalidixic acid • Differential • Hemolysis

  12. MSA results Selective 7.5% NaCl Staphylococcus sp Differential Mannitol fermentation phenol red  yellow

  13. MAC results • Selective: GN • CV • Bile Salts • Differential • Lactose fermentation • NF = colorless • Coliforms • Neutral red  pink

  14. EMB results • Selective: GN • Dyes • Eosin • MB • Differential • Lactose fermentation • NF = colorless • Acid production • Small = pink • Large = metallic green

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