1 / 5

Isopropylmalate Synthase

This study explores the effects of amino acid analogs, specifically fluoroleucine, on wild-type Salmonella and their growth on minimal media. Isopropylmalate synthase, the first committed enzyme in leucine biosynthesis, is analyzed to understand why normal amino acids are not synthesized and what occurs when analogs are incorporated into proteins. The nature of mutant variants that can grow in the presence of fluoroleucine is examined, providing insights into bacterial adaptability and metabolic pathways.

feleti
Télécharger la présentation

Isopropylmalate Synthase

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Isopropylmalate Synthase Amino acid analogs

  2. Amino Acid Analogs Analogs – resemble naturally occurring amino acids Inhibit Wild Type – Why? What happens if incorporated into protein? Why are normal amino acids not synthesized?

  3. Expose Wild Type Salmonella to Fluoroleucine Observed Expected Minimal Media containing Fl-leu What is nature of variants that grow?

  4. Salmonella Strain ComparisonGrown on regular Minimal Media Isopropyl Malate Synthase – First committed enzyme in the synthesis of leucine

  5. Nature of Mutants

More Related