1 / 2

Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit

Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit. HC 70AL SPRING 2009 APRIL 7, 2009 ANHTHU BUI. Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit. SRB Leaves Freezed in liquid nitrogen. Lysate. Adjust buffer conditions by

felicia-munoz
Télécharger la présentation

Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit HC 70ALSPRING 2009APRIL 7, 2009ANHTHU BUI

  2. Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit SRB Leaves Freezed in liquid nitrogen Lysate Adjust buffer conditions by adding AP3 buffer &ethanol Grind into powder with a mortar & pestle Separate DNA from contaminants using a silica-gel spin column Frozen SRB Leaf Powder Lyse cells with AP1 extraction buffer Genomic DNA, ribosomal RNAs, tRNAs, mRNAs, proteins, polysaccharides Genomic DNA bound to silica-gel column Digest RNAs with RNase A Wash column Centrifuge Centrifuge Elute with water or low-salt buffer Genomic DNA, proteins, oligo-ribonucleotides, polysaccharides Cell debris Pure genomic DNA Precipitate polysaccharides & proteins with AP2 buffer Filtered & homogenized via Shredder column Lysate

More Related