1 / 10

Mycobacterium tusciae , sp. nov.

Mycobacterium tusciae , sp. nov. Enrico Tortoli. 19° ESM, Lisbon, July 22-25, 1998. Case report. 7-year old boy suffering from nephrotic syndrome from the age of 3 steroid treatment in the last 4 years cervical lymphadenitis surgical removal no relapse so far. Cultural features.

flo
Télécharger la présentation

Mycobacterium tusciae , sp. nov.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Mycobacterium tusciae, sp. nov. Enrico Tortoli 19° ESM, Lisbon, July 22-25, 1998

  2. Case report • 7-year old boy suffering from nephrotic syndrome from the age of 3 • steroid treatment in the last 4 years • cervical lymphadenitis • surgical removal • no relapse so far

  3. Cultural features • growth: slow (4 weeks) • growth temperature: 25°-32°C • pigmentation: scotochromogenic • colonies: rough

  4. Biochemical features • positive reactions: • nitrate reduction • thermostable catalase • urease • Tween 80 hydrolysis • 14-day arylsulfatase • acid phosphatase

  5. Antimicrobial susceptibility Drug MIC Ciprofloxacin  1 Clarithromycin  2 Ethambutol 8 Rifabutin  0.5 Rifampin  0.5 Sparfloxacin  0.5 Streptomycin  2

  6. Lipidic analysis • TLC: • -mycolates • keto-mycolates • wax esters • pattern shared by: M. scrofulaceum, M. interjectum, M. xenopi • Gas-chromatography: • 16:0 30% • 18:1 cis 9 21% • 18:0 alcohol 11% • 18:0 10-methyl 10% • pattern shared by M. scrofulaceum

  7. lmwIS hmwIS M. intracellulare FI-25796 HPLC

  8. 16S rDNA sequence • unique sequence both in hypervariable region A and B • short helix 18 • phylogenetic position among rapid growers, close to M. flavescens and M. smegmatis

  9. M. tusciae sp. nov. • Features of the type strain • acid fast rod • G + C content = 66.4 mol% • distinguishable from other scotochromogenic mycobacteria by means of biochemical tests • unique HPLC profile • not identifiable by TLC and GC alone • susceptible to all antimycobacterial drugs • unique sequence within 16S rDNA

  10. Conclusions • M. scrofulaceum is classically considered a major responsible of cervical lymphadenitis • M. tusciae adds to the scotochromogenic mycobacteria recently described as cause of pediatric lymphadenitis • part of pathologies attributed to M. scrofulaceum are probably due to misidentified novel mycobacteria

More Related