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The V. fischeri Autoinducer

The V. fischeri Autoinducer. N -( b -ketocaproyl)- L -homoserine lactone. LuxI. Quorum Sensing. LuxR. LuxR. target gene. Acyl-Homoserine Lactone Autoinducers. V. fischeri P.aeruginosa A. tumefaciens E. carotovora. Density dependent luminescence Virulence factors

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The V. fischeri Autoinducer

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  1. The V. fischeri Autoinducer N-(b-ketocaproyl)-L-homoserine lactone

  2. LuxI Quorum Sensing LuxR LuxR target gene

  3. Acyl-Homoserine Lactone Autoinducers

  4. V. fischeri P.aeruginosa A. tumefaciens E. carotovora Density dependent luminescence Virulence factors Conjugal transfer of Ti plasmid Antibiotic synthesis LuxI-LuxR Quorum Sensing Systems

  5. LuxP LuxR Quorum Sensing in V. harveyi AI-2 LuxQ H D H D HTH LuxU LuxO H D LuxN AI-1 LuxCDABE

  6. Quorum Sensing in E. coli and Salmonella

  7. Bacillus anthracis Bacillus halodurans Bacillus subtilis Borrelia burgdorferi Campylobacter jejuni Clostridium acetobolyticum Clostridium difficile Clostridium perfringens Deinococcus radiodurans Escherichia coli Enterococcus faecalis Haemophilus influenzae Helicobacter pylori Klebsiella pneumoniae Lactococcus lactis Leuconostoc oenos Listeria monocytogenes Neisseria gonorrhoeae Neisseria meningitidis Pasteurella multocida Porphyromonas gingivalis Proteus mirabilis Salmonella paratyphi Salmonella typhi Salmonella typhimurium Shewanella putrefaciens Staphylococcus aureus Staphylococcus epidermidis Streptococcus gordonii Streptococcus mutans Streptococcus pneumoniae Streptococcus pyogenes Vibrio anguillarum Vibrio cholerae Vibrio harveyi Vibrio vulnificus Yersinia pestis Bacteria That Contain luxS

  8. luxS metK pfs

  9. MetK Pfs Pfs Page544

  10. SAM Utilization Met MetK SAM SAH MTA Pfs Pfs SRH MTR 4,5-Dihydroxy- 2,3-pentanedione + HC ?

  11. SAM Utilization Met MetK SAM SAH MTA Pfs Pfs SRH MTR LuxS? 4,5-Dihydroxy- 2,3-pentanedione + HC ?

  12. SAM Utilization Met MetK SAM SAH MTA Pfs Pfs SRH MTR LuxS AI-2 + HC ?

  13. The LuxS Reaction 4,5-Dihydroxy- 2,3-pentanedione SRH + Homocysteine

  14. AI-2 Could be a Furanone MHF DMHF HF DHCP

  15. LuxP LuxR LuxCDABE Quorum Sensing in V. harveyi AI-2 LuxQ H D H D HTH LuxU LuxO H D LuxN AI-1

  16. Holo-LuxP

  17. Boron is ubiquitous in the biosphere Sea water 0.4 mM (primarily boric acid) L.A. tap water 40 μM Soils 2-100 ppm Marine plants 120 ppm Land plants 50 ppm Marine animals 20-50 ppm Land animals 0.5 ppm Boron is required by a number of organisms, but for poorly characterized reasons Vascular plants cell wall? pollination? Diatoms Cyanobacteria heterocyst formation Zebrafish embryogenesis

  18. 11BNMR holo-LuxP filtered apo-LuxP

  19. Synthesis of AI-2

  20. Boric Acid Induces Lux in V. harveyi

  21. Synthesis of AI-2

  22. Targets of AI-2 Vibrio harveyi Bioluminescence, Biofilms Vibrio cholerae Virulence, Biofilms Salmonella typhimurium ABC transporter Salmonella typhi Biofilms Escherichia coli O157:H7Type III Secretion (virulence) Porphyromonas gingivalis Hemin acquisition genes, virulence factors Shigella flexneri VirB (virulence factor) Streptococcus pyogenes Secreted protease Actinobacillus actinomycetemcomitans Periodontal virulence, Mixed-species biofilms Photorhabdus luminescens Antibiotic production Neisseria meningitidis Virulence/bacteremia Borrelia burgdorferi Virulence Clostridium perfringens Virulence Campylobacter jejuni Motility Microarrays Escherichia coli K12 ~300 genes Escherichia coli O157:H7 ~400 genes Vibrio cholerae El Tor ~70 genes (virulence regulon)

  23. Biosynthesis of AI-1 and AI-2 Met MetK LuxI SAM LuxLM AI-1 SAH MTA Pfs Pfs SRH MTR LuxS AI-2 + HC ?

  24. LuxP LuxR LuxCDABE Quorum Sensing in V. harveyi AI-2 LuxQ H D H D HTH LuxU LuxO H D LuxN AI-1

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