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Understanding the Repressilator: A Milestone in Synthetic Biology

In 2000, Stanislas Leibler and Michael Elowitz developed the Repressilator, a groundbreaking genetic machine designed to function as an oscillatory system, demonstrating the principles of synthetic biology. This genetic clock operates using E. coli and utilizes regulated promoters and repressors to create synchronized oscillations. Despite its innovation, the system faces challenges such as losing synchronization and environmental noise. This endeavor lays the foundation for future advancements in genetic engineering and synthetic networks, though its practical application remains limited.

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Understanding the Repressilator: A Milestone in Synthetic Biology

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  1. REPRESSILATOR SILAS FERRAO

  2. WHAT IS IT? • IN 2000 STANISLAS LIEBLER AND MICHAEL ELOWITZ CREATED GENETIC MACHINE • CALLED REPRESSILATOR-MILESTONE IN SYNTHETIC BIOLOGY, IT WAS AN OSCILLATORY GENTIC MACHINE • USED E. COLI TO EXHIBIT REGULATORY NETWORK

  3. HOW DOES IT WORK? • “GENETIC CLOCK” • TETR, LACL, λCL ARE REPRESSORS • PTET, PLAC, λPR ARE PROMOTERS • ARRANGED ON PLASMID ALONGSIDE GFP PLASMID

  4. HOW DOES IT WORK? LACL L-PR

  5. PROBLEMS….. • OSCILLATIONS LAST FOR TWO AND A HALF HOURS • LOSE SYNCH WITHIN SYSTEM • SYSTEM IS “NOISY”

  6. DEVICE…. CELIAC DISEASE I HATE IT WHEN THIS HAPPENS TO ME…

  7. WHAT IS IT? • AUTOIMMUNE DISORDER IN SMALL INTESTINE • DAMAGES THE VILLI (ATROPHY) • REACTION TO GLIADIN

  8. GLIADIN • GROUP OF PROTEIN FOUND IN GLUTEN • A-GLIADIN • GLUTEN HALF OF THE WHEAT SEED

  9. DEVICE Cyclic AMP CR CXCR3 R2-HMW Chromosome GLN BP Plasmid H. PYLORI RHO TERM. HD RBS E. COLI ORF A-gliadin promoter

  10. HD RBS

  11. RESULTS…. • VERY IMPRACTICAL • GLUTEN ANGLE • RECEPTORS, TERMINATORS, BACTERIA ONLY TO DEAL WITH INHBITING GLUTEN CROSSLINKING

  12. THANK YOU DOE!

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