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adapted

adapted. unadapted. F 1. F 2. F 3. Jeong Im Kim Hyong Gong Mang Ray Bressan Matthew Jenks Dae-Jin Yun Purdue University WCU Program, Gyeongsang, Korea. Col-0. rst1-2. C24. rst1-1. BG1. Glycosyl hydrolase family 1 (BG1). Tubulin. 5 weeks. 4 weeks. 6 weeks. 6 weeks. a. c. d.

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adapted

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  1. adapted unadapted F1 F2 F3

  2. Jeong Im Kim Hyong Gong Mang Ray Bressan Matthew Jenks Dae-Jin Yun Purdue University WCU Program, Gyeongsang, Korea

  3. Col-0 rst1-2 C24 rst1-1 BG1 Glycosyl hydrolase family 1 (BG1) Tubulin 5 weeks 4 weeks 6 weeks 6 weeks a c d b

  4. The rst mutant/phenotypes can reverse to wt phenotype and back to mutant even when the rst locus is still homozygous mutant • The rst mutation causes epigenetic (non sequence) change in DNA that produces rst phenotypes (hypothesis)

  5. * * * * * yucca6-2D yucca6-1D WT WT YUCCA6 ACTIN A yucca6-1D yucca6-2D yucca6-ko B NOS-T CaMV35S yucca6-OX C

  6. A C 20 week-old yucca6-1D Arabidopsis staygreen D B Sorghum staygreen variety Wheat staygreen variety

  7. WT yucca6 -2D Vector T8 (35S:YUC6)

  8. Lys Lys IAA iaaL IAA 35S::iaaL transgenic plant: Over-expressingiaaL(Indole acetic acid-Lysine synthetase)gene driven by 35S CaMV promoter iaaL(Indole acetic acid-Lysine synthetase) from Pseudomonas savastanoi, not found in plants

  9. Conclusions: • YUCCA6 controls unique phenotypes not controlled by other YUCCA gene family members. • 35S YUCCA6 overexpression recapitulates all phenotypes of the original YUCCA6 OX mutant and multiple OX alleles of YUCCA6 have the same phenotype: therefore, YUCCA6 is the correct locus involved in these phenotypes. • Removal of the increased auxin resulting from overexpression of YUCCA6 does not remove all of the phenotypes resulting from YUCCA6 overexpression, e.g. staygreen. • Therefore, some phenotypes like staygreen are not directly the result of auxin overproduction but appear to be related somehow to ROS levels.

  10. Control #4 #46

  11. SCIENCE (the scientific method) • Observation • Hypothesis • Experiment/Test (surveys and computers only) are out • Repeat • Then we confuse systematic for scientific

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