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F plasmid integrates into chromosome by recombination

Donor chromosome. F + cell. 1. F plasmid integrates into chromosome by recombination. Hfr cell. Donor chromosome. F + cell. 1. F plasmid integrates into chromosome by recombination. Hfr cell. Pilus. 2. Cells join via a conjugation pilus. F + cell. F – recipient. Donor chromosome.

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F plasmid integrates into chromosome by recombination

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  1. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell

  2. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient

  3. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient Copy of donor DNA F plasmid Part of F plasmid 3 F plasmid partially replicates and moves into recipient cell trailing a copy of donor’s DNA

  4. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient Copy of donor DNA F plasmid Part of F plasmid 3 F plasmid partially replicates and moves into recipient cell trailing a copy of donor’s DNA Incomplete F plasmid; cell remains F– 4 Conjugation ends with pieces of F plasmid and donor DNA in recipient cell Hfr cell F+ cell

  5. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient Copy of donor DNA F plasmid Part of F plasmid 3 F plasmid partially replicates and moves into recipient cell trailing a copy of donor’s DNA Incomplete F plasmid; cell remains F– 4 Conjugation ends with pieces of F plasmid and donor DNA in recipient cell Hfr cell F+ cell 5 Donor DNA and recipient DNA recombine making a recombinant F– cell Recombinant cell

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