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Characterizing Growth of Caffeine-Transforming Cells in Tetracycline Broth

This project aims to enhance our understanding of cell growth in tetracycline broth, focusing on the specific growth of JM109 #23 cells that convert caffeine to theophylline. Recent experiments, including incubation at 37°C for one day followed by three days at room temperature, have shown promising results. The study will continue by evaluating various caffeine concentrations and exploring growth patterns of the ΔNEP1 genotype alongside different genetic combinations. Collaborators include Shannon Doherty, Erika Keffeler, and Jon Lim.

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Characterizing Growth of Caffeine-Transforming Cells in Tetracycline Broth

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Presentation Transcript

  1. Characterizing growth in broth Planning for August 2014 and onward

  2. Current Project Status • Interesting results from summer experiments • Goal: grow cells that convert caffeine to theophylline in tetracycline broth • August: replicate Shannon Doherty’s growth of JM109 #23 in broth • One day @ 37°C and three days @ room temperature • Pilot study for incubation only at RT • Control for tetracycline function • Characterize growth of ΔNEP1:#23 cells Shannon Doherty, Erika Keffeler, Jon Lim

  3. Looking forward • Goal: expand knowledge on growth in tetracycline broth • Continue growing cells in various concentrations of caffeine • Grow cells exclusively at RT • Investigate growth of ΔNEP1 with other genotype combinations Shannon Doherty, Erika Keffeler, Jon Lim

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