1 / 13

TWIST and Senescence

TWIST and Senescence. Emily McMillan 1/22/08. Differentiation. Transcription factor bLHL Proliferation factor Binds RUNX2/CBFA1 Inhibits osteoblast differentiation. Rose and Malcom. 1997 TIG: 384. Aging. TWIST differentially expressed Young fibroblasts = ↑ TWIST1

glenda
Télécharger la présentation

TWIST and Senescence

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. TWIST and Senescence Emily McMillan 1/22/08

  2. Differentiation • Transcription factor • bLHL • Proliferation factor • Binds RUNX2/CBFA1 • Inhibits osteoblast differentiation Rose and Malcom. 1997 TIG: 384.

  3. Aging • TWIST differentially expressed • Young fibroblasts = ↑ TWIST1 • Senescent fibroblasts = ↓ TWIST1

  4. Do senescent fibroblasts have an osteoblast-like phenotype?

  5. Cell Types • WI-38 (lung fibroblasts) • AG13153 (skin fibroblasts) • NHOst • U2Os (osteosarcoma) • BMSC (+/- differentiation factors)

  6. Gene Markers • Twist • Beta-2-microglobin (normalization) • Osteogenic Markers • Runx2 • Osterix • Osteocalcin • Osteopontin • Osteonectin • Bone Sialoprotein • Alkaline Phospatase • Collagen Type 1A

  7. Analyses • Real-Time PCR • Immunofluorescence • Western blot

  8. Northern vs. Real-Time Wang et al (1996)

  9. RUNX2 Levels

  10. Collagen 1A Levels

  11. Osteonectin Levels

  12. Osteopontin Levels

More Related