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Function and Structure of the Cytochrome bc1 Complex

Explore the function and structure of the Cytochrome bc1 complex, known as ubiquinol:cytochrome c oxidoreductase. Learn about the proton pump mechanism, Qo site, Reiske Iron Sulfer Protein, and electron transfer in this integral biological system. For more information, visit Prof. Crofts' bc1 Complex Site.

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Function and Structure of the Cytochrome bc1 Complex

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  1. bc1 complex Stuart Rose

  2. Goal of this presentation: • Function of bc1 complex • Overall Structure • Structural Details Related to Function

  3. bc1 complex function • Cytochrome bc1 complex or • ubiquinol:cytochrome c oxidoreductase • “Proton pump”: proton translocation coupled to electron transfer FOR MORE INFO... Prof. Crofts’ bc1 Complex Site http://www.life.uiuc.edu/crofts/bc-complex_site/

  4. Full Dimer Two monomers with two fold symmetry. Shown here generally colored by chain designation and axis of symmetry located along a vertical line the image plane.

  5. Functional Highlights: Qo Site • Qo site: oxidation of quinol, QH2,to quinone, Q • Conformation of site: Liganding of Q within pocket and Liganding of different inhibitor types within pocket • Movement of Reiske Iron Sulfer Protein (ISP) between two domains: oxidation of quinol to reduction of cytochrome c1 at heme propianate of the cytochrome

  6. Schematic view of Q-cycle:

  7. bc1 complex subunits of interest

  8. Reiske (ISP) Binding Domains Cyt b ISP Cyt c1

  9. Electron Transfer FromISP to cyt c1 and cyt b

  10. Qo Site with Stigmatellin

  11. Quinone, Q, vs Stigmatellin Oxidized by ISP Reduces bl Quinol, QH2, orthographic view ISP interaction Interference with proximate zone Stigmatellin, perspective view

  12. Distances between redox centers proximal to Qo-site

  13. an electrons view….

  14. Ligation of hemes in Cyt b

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