JOINT RESEARCH AGREEMENTAIST - Biomass Technology Research Center and UFRJ – Chemistry Institute 01/07/2008
COLLABORATION AREA Research on Sustainable Biofuel Production from Lignocellulosic Biomass Resources 01/07/2008
PRINCIPLES • Sustainable zero-carbon emission total system of biofuels production • No competition with food production • Bioethanol from lignocellulosic biomass resources • Mainly from sugarcane biomass residue (bagasse) • Using non- acidic pretreatments • Enzymatic hydrolysis • Ethanol fermentation
Estimate for 2008 (April 29th) • Biggest production ever From 607.8 to 631.5 million tons of sugarcane will be harvested Brazil will produce 26.4 - 27.4 billion liters of ethanol 4.2 billion liters will be exported (2.5 billion liters to the USA) Figures are 14.9% to 19.4% higher in comparison to 2007 Source: www.conab.gov.br
Biomass Ethanol
Sugarcane Plantation MechanizedStraw Haverst Non mechanized
Ethanol from Biomass Residue Straw and/or Bagasse ETHANOL
ENZYMATIC Saccharification Buffer treated corn stover Enzyme treated corn stover Acknowledgement NREL - USA
Bagasse productionandavailability/year (kg) Theoretical ethanol yield from sugarcane biomass cellulose
ETHANOL PRODUCTION VIA ENZYMATIC HYDROLYSIS OF SUGAR-CANE BAGASSE AND STRAW BRAZILIAN BIOETHANOL PROJECT Elba P. S. Bon - Scientific Coordinator Chemistry Institute Federal University of Rio de Janeiro - Brazil elba1996@iq.ufrj.br 01/07/2008
The BIO-ETHANOL Project - Synopsis RESEARCH NETWORK to develop in Brazil the technology for the conversion of the sugarcane biomass (bagasse and straw) into fuel ethanol using enzymatic hydrolysis.
Main Research Areas • Development of biomass pre-treatment processes for sugar cane bagasse and straw • Raw and pre-treated biomass characterization • Cellulases / xylanases production • Enzymatic hydrolysis • Sugars syrups characterization • Ethanol fermentation (C6) • C5 sugars and lignin uses • Energy optimisation • Effluents and water
Process Overview Pre-treatment • Steam explosion • Milling Pre treatment Sugarcane biomass Enzymatic hydrolysis Harvest Fermentation Destilation Ethanol Enzyme Production Trichoderma reesei RUT C30 and Aspergillus awamori
Enzymes Production Area
Principles • Enzyme cost contribution and effectiveness depends on the biomass source and pre-treatment conditions • Development of “tailored made” enzyme blends for sugarcane biomass • Use of crude “cellulase/xylanase /accessory enzymes” preparations • “In house” production to reduce cost
Sugarcane bagasse enzymatic hydrolysis using Trichoderma reesei cellulase
BIOMASS Sugarcane Bagasse Treated Sugarcane Bagasse
HYDROLYSIS RESULTS Hydrolysis of STEAM TREATED bagasse in presence of an ACID CATALYST – Lund University (STBA - 53% cellulose) FPU/BGU LAC: 1 CG 220: 0,70 Spezyme CP: 0,75 HYDROLYSIS EXPERIMENTS Volume: 100 mL Bagasse conc.: 25 g/L ENZYME LOAD: 10 FPU/G • ENZYMES • GC 220 (Genencor) • Spezyme CP (Genencor) • Produced on lactose (LAC)
Preliminary hydrolysis experiments using milled Eucalyptus provided by the AIST Biomass Research Centre JAPAN
HYDROLYSIS EXPERIMENTS USING Eucalyptus
MILLING DOES NOT • USE WATER, HIGH TEMPERATURE OR PRESSURE • ALTER THE BIOMASS COMPONENTS CHEMICALLY (ADVANTAGE FOR BIOREFINARY) • GENERATE INHIBITORS FOR THE HYDROLYSIS AND FERMENTATION STEPS • GENERATE POLLUTANT WATER STREAMS AND SALT • MAY BE EASIER TO SCALE UP IN COMPARISON TO STEAM EXPLOSION • IT IS SAFER TO OPERATE • ENERGY CONSUMPTION AND MAINTENANCE ARE THE SENSITIVE ASPECTS
MILLED EUCALYPTUS HYDROLYSIS RESULTS • Milled Eucalyptus particle size: 25 micrometer • Eucalyptus contains 42% of cellulose • Hydrolysis: biomass 25g/L, 10 FPU/g, 50ºC, 200 rpm • Hydrolysis yields: 98% using the ENZYTEC blend and 92% using the Acremonium enzyme • Faster process using the ENZYTEC enzyme
HYDROLYSIS RESULTS Hydrolysis of STEAM TREATED bagasse in presence of an ACID CATALYST – Lund University (STBA - 53% cellulose) FPU/BGU LAC: 1 CG 220: 0,70 Spezyme CP: 0,75 HYDROLYSIS EXPERIMENTS Volume: 100 mL Bagasse conc.: 25 g/L ENZYME LOAD: 10 FPU/G • ENZYMES • GC 220 (Genencor) • Spezyme CP (Genencor) • Produced on lactose (LAC)
Sugarcane biomass hydrolysis using the ENZITEC enzyme blend
HYDROLYSIS EXPERIMENTS (STBA – 53% cellulose) • 130 g/L of treated sugarcane bagasse • ENZITEC blend – 10 FPU/g • Sodium citrate buffer pH 4.8 • Temperature - 50ºC • Agitation - 200rpm
STBA HYDROLYSIS RESULTS Glucose Syrup 60 g/L (87%Yield) Ethanol fermentation Lignin STBA + ENZITEC Enzyme Solid hydrophobic fuel
Obrigada!
