1 / 1

B2 repeat

B2 repeat. PGN. pA. 3’ LTR. SA. enh. = Trap-specific nested primers = B2-specific primer = Suppression sequence. B2 repeat. B2 repeat. Gentile et al., Supplementary Figure 2

gur
Télécharger la présentation

B2 repeat

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. B2 repeat PGN pA 3’ LTR SA enh = Trap-specific nested primers = B2-specific primer = Suppression sequence B2 repeat B2 repeat Gentile et al., Supplementary Figure 2 RAN-PCR procedure for mapping trap integration sites. Specific amplification of genomic regions comprised between a B2 repeat and an integrated trap occurs by means of a B2-specific primer and two nested primers designed on the trap cassette, as indicated. A Suppression sequence added 5’ to the B2 primer avoids aspecific B2-B2 amplification by promoting the formation of panhandle-like structures which cannot be amplified, as illustrated in the lower part of the figure.

More Related