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Culture Media

Culture Media. Prof. Dr. Ihab Moussa. Culture Media. Indications/ Need for culture - Isolate bacteria in pure cultures. Demonstrate their properties. Obtain sufficient growth for preparation of antigens & for other tests. Typing bacterial isolates. Antibiotic sensitivity.

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Culture Media

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  1. Culture Media Prof. Dr. Ihab Moussa

  2. Culture Media Indications/ Need for culture - • Isolate bacteria in pure cultures. • Demonstrate their properties. • Obtain sufficient growth for preparation of antigens & for other tests. • Typing bacterial isolates. • Antibiotic sensitivity. • Estimate viable counts. • Maintain stock cultures.

  3. Classification of Media • Based on the consistency : Solid media Semisolid media Liquid media

  4. Classification • Based on the function or property: - Simple media - Enriched media - Enrichment broth - Selective media - Indicator media - Differential media - Composite media - Transport media

  5. Constituents Of Media • Agar or agar- agar • Peptone - mixture of partially digested proteins • Yeast or meat extract • NaCl • Melting point : 98°C • Solidifying point : 42°C • % of agar : Solid media (2%)

  6. Simple Media • Also called Basal medium • Most commonly used in routine labs. e.g. Nutrient broth, NA

  7. Enriched Media • Blood, serum or egg added to the basal medium. • To grow bacteria which are more exacting in nutritional requirements. e.g. Blood Agar, Chocolate Agar

  8. Blood Agar Media

  9. Enrichment Broth (media) • For mixed cultures or materials containing more than one bacterium. • Contains substances which stimulates wanted bacteria & inhibits unwanted bacteria. e.g. Tetrathionate broth

  10. Selective Media • Substances added to a solid media to favour the growth of wanted bacteria. e.g. MacConkey agar media

  11. Indicator Media • Change in the colour of indicator with the growth of bacteria. e.g. Sulphite in Wilson Blair media for Salmonella typhi.

  12. Differential Media • To bring out differing characteristics of bacteria. e.g. MacConkey’s agar

  13. Composite Media • For identification of isolates e.g. Triple Sugar Iron Transport Media • For delicate organisms • Delay in transit e.g. Stuart’s transport media Dr Ekta, Microbiology, GMCA

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