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Loose Connective Tissue

Loose Connective Tissue. ( LCT ). Areolar Tissue Pink = collagen Purple = elastin. Orecin Stain for Elastin – purple “ribbon candy”. Sliver Stain for Reticular Fibers. Collagen synthesis and organization. PAS stain of epithelial basement membranes (BM). Cells of the LCT. Fibroblasts.

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Loose Connective Tissue

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  1. Loose Connective Tissue (LCT)

  2. Areolar Tissue Pink = collagen Purple = elastin

  3. Orecin Stain for Elastin – purple “ribbon candy”

  4. Sliver Stain for Reticular Fibers

  5. Collagen synthesis and organization

  6. PAS stain of epithelial basement membranes (BM)

  7. Cells of the LCT Fibroblasts

  8. LM of fibroblasts (arrows)

  9. Adipose and Adipocytes

  10. Adipocytes (fat cells)

  11. Adipose Tissue – fat cells + CT septa (arrows)

  12. Adipose Tissue fixed and stained w/ Osmium Tetroxide

  13. Defense cells of the LCT Neutrophils, Plasma cells, Mast cells, and Macrophage

  14. LCT – a = pericytes; b = mast cell; c = plasma cells; d = capillary

  15. LCT – M = mast cell; P = plasma cells; F = fibroblasts; Eo = eosinophils, N = neutrophils

  16. TEM of a Plasma Cell

  17. TEM of a Mast Cell

  18. TEM of a Macrophage

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