Thayer - Martin agar - PowerPoint PPT Presentation

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Thayer - Martin agar

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  1. Thayer - Martin agar • Chocolate agar has been modified to be selective for Neisseria gonorrhoeaeand Neisseria meningitidisby the addition of antibiotics, (V-C-N inhibitor) including: • Colistin: to inhibit most gram negative bacteria other than Neiseria. • Vancomycin: to inhibit most gram positive bacteria. • Nystatin or anisomycin :to inhibit yeast. • Modified Thayer - Martain agar includes trimethoprim to inhibit Proteus.

  2. Neisseria gonorrhoeae Typical colonial morphology on Thayer-Martin Selective Agar of Neisseria gonorrhoeae .... Small, grayish-white to colorless, mucoid

  3. Jembec plate In the JEMBEC system, a tablet consisting of a mixture of citric acid and sodium bicarbonate is placed in a well within the plate and is activated by the moisture (humidity) produced by the culture medium within the sealed plastic bag. The CO2 levels generated are sufficient for the growth of Neisseria gonorrhoeae on the selective medium provided with the system

  4. Neisseria gonorrhoeae Gram stain

  5. Direct Immuno fluorescence (DIF) Antibody labeling for Neisseria gonorrhoeae

  6. Direct Immuno fluorescence (DIF) Antibody labeling for Chlamydia trachomatis

  7. Chlamydia trachomatis With Giema stain With Iodine stain Giemsa stain of Chlamydia inclusion bodies (purple "caps" on epithelial cell).

  8. Post specimen processing • Interfering factors: • Patient on antibiotic therapy. • Improper sample collection. • Result reporting: • Report Gram stain finding as an initial report. • Report the isolated and its sensitivity pattern as a final report. • Turn around time: • Gram stain result should be available half hour after specimen receipt. • Isolation of a possible pathogen can be expected after 2-3 days. • Negative culture will be reported out 1-2 days after the receipt of the specimen.