1. Structural Evidence for Evolution of the β/α Barrel Scaffold by Gene Duplication and Fusion Horng –uei Lii

2. Background What is β/α Barrel ?

3. β/α Barrel (I) • Are found in many proteins(enzymes) • The eight-stranded β/α Barrel structure are one of the largest and most regular of all domain structure

4. β/α Barrel(II) • Different structure and function • Loops---varies, functional domain • Evolution material

6. TIM barrel Triosephosphate isomerase

7. Introduction • Evolution Material • Computational Tool • Genome Protein Dtata

8. Materials Thermotoga maritima HisA & HisF

9. HisA Converts (ProFAR) to (PRFAR)

11. HisF Catalyze condensation of PRFAR and ammonia,  AICAR and ImGP HisF:HisH cowork

12. Methods • X-ray analysis • Multi-sequence alignment • Superimpose • Mutual residual activities

17. Conclusion • Half-barrel Ancestor • Duplication and Fusion • Model

19. Application Subdomain structures: Genome search Functionally and structurally related proteins

21. Thank you!