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Albumin Analysis by Potentiometric Method

Albumin Analysis by Potentiometric Method. The Advantages of Potentiometric Method. 1. Integrate easily 2. Microfabricate easily 3. Real time response. Experimental. Materials. Monomer 3-Dimethylaminopropyl methacrylamide:DMAPMA Crosslinking agent

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Albumin Analysis by Potentiometric Method

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  1. Albumin Analysis by Potentiometric Method

  2. The Advantages of PotentiometricMethod • 1. Integrate easily • 2. Microfabricate easily • 3. Real time response

  3. Experimental

  4. Materials • Monomer • 3-Dimethylaminopropyl methacrylamide:DMAPMA • Crosslinking agent • Tetraethylene glycol dimethacrylate:TEGDMA

  5. Materials • Initiators • Cumene hydroperoxide:CHP • Tetramethyl ethylenediamine:TMEDA • Electrodes • Working electrode: Graphite, Au, Ag • Counter electrode: Pt • Reference electrode: Ag/AgCl • Electrolyte:0.5M KCl

  6. Stabilization of electrode potential in open circuit Surface cleaning of electrode surface Determine V of electrode after albumin addition Linear regression of V and albumin concn. Experimental Sequence of Potentiometric Method

  7. Extend by spin coating and UV curing Prepare polymerization reaction solution Washing with 20 wt.% methanol and drying MIP Determine potential change by potentiostat Fabrication Sequence of MIP thin film

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