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Reproducibility of Electrochemical Measurements Using Potentiometric Methods for Albumin Analysis

This study assesses the reproducibility of peak current responses from gold wire electrodes in potentiometric methods for albumin analysis. Cyclic voltammograms were collected using a CHI-405 potentiostat across a specified voltage range with various wash solutions for electrode treatment. The results demonstrate significant reproducibility in the peak currents when electrodes are treated traditionally and with alternate methods. The findings contribute valuable insights into optimizing electrochemical analysis for biomolecules.

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Reproducibility of Electrochemical Measurements Using Potentiometric Methods for Albumin Analysis

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  1. Albumin Analysis by Potentiometric Method

  2. Table Ⅳ:The peak current reproduciblity of electrode treated by traditional method

  3. Fig 6. The cyclic voltammogram of Au wire electrode WE: Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: 0.5 M KCl 50 ml(20 mM K4Fe(CN)6+20 mM K3Fe(CN)6), Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec

  4. Table Ⅴ: The peak current reproduciblity of electrode treated by traditional method

  5. Fig 7. The cyclic voltammogram of Au wire electrode WE: Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: wash soiution, Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec

  6. Fig 8. The cyclic voltammogram of Au wire electrode WE: Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: 0.5 M KCl 50 ml(20 mM K4Fe(CN)6+20 mM K3Fe(CN)6), Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec, Wash method: 10min.1N NaOH+10min. 1N HCl+10min.1N HNO3

  7. Table Ⅵ: The peak current reproduciblity of electrode treated by traditional method

  8. Fig 3. The cyclic voltammogram of selectivity WE: Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: 0.5 M KCl 50 ml(20 mM K4Fe(CN)6+20 mM K3Fe(CN)6), Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec

  9. TableⅡ: The peak current of selectivity

  10. Fig 9. The cyclic voltammogram of Au wire electrode WE: Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: wash solution, Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec, wash method: 3min. Boiling water+1min.ultrasonic

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