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lacZ lacY lacA

lacZ lacY lacA. lacZ lacY lacA. lacZ lacY lacA. lacZ lacY lacA. lacZ lacY lacA. A. A. A. A. O. O. O. O. A. O. i. i. i. i. i. Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

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lacZ lacY lacA

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  1. lacZ lacY lacA lacZ lacY lacA lacZ lacY lacA lacZ lacY lacA lacZ lacY lacA A A A A O O O O A O i i i i i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex NO transcription Transcription occurs NO transcription Lac operon operator Regulatory gene activator P B-galactosidase lactose permease transcetylase

  2. Glucose glycolysis PEP ATP glucose Pyruvate IIA HPr EI IIC Kreb’s IIB glucose Low cAMP lactose Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex Lots of glucose Not taken up

  3. Glucose PEP Pyruvate IIA HPr EI Kreb’s This process slows down glycolysis ATP Not more glucose IIC IIB Adenylate cyclase Levels of cAMP increase lactose Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex

  4. Glucose PEP Pyruvate IIA HPr EI Kreb’s This process slows down glycolysis ATP Not more glucose IIC IIB Adenylate cyclase lacOp becomes active lactose Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex

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