raw image

1. a) b) raw image Histogram of confidence measure Histogram of confidence measure Correction, segmentation Histogram of confidence measure Histogram of confidence measure segmented image Biological feature quantification Robust regression Histogram of final confidence measure c) ellipse fits Red/green normalization clustering Cell-shape, type heuristics cell-type, stage assignment Cell confidence Typical object Typical object Typical object Typical object Typical object Typical object Typical object Typical object

2. Expression intensity a) b) Distance between Distance to cell periphery Distance to cell centre Distance to pattern centre bud mother CSC24 SLD3 PCL8 CDC6 ASH1 SIC1 daughter lone cell daughter lone cell daughter lone cell daughter lone cell mother mother mother mother bud bud bud bud PDR12 RSN1 TCB1 FTR1 MID2 TCB3 TCB2 YGR026W MRH1 HXT6 SNQ2 MEP1 CDC6 bud CDC6 bud CDC6 bud CDC6 mother CDC6 mother CDC6 mother CDC6 SIC1 bud SIC1 bud SIC1 bud SIC1 mother SIC1 mother SIC1 mother SIC1 ASH1 bud ASH1 bud ASH1 bud ASH1 mother ASH1 mother ASH1 mother ASH1 FTR1 bud FTR1 mother FTR1 daughter FTR1 lone cell FTR1 HXT6 bud HXT6 mother HXT6 daughter HXT6 lone cell HXT6

3. c) Distance to cell periphery Distance to cell centre bud mother bud mother STB1 WHI5 MCM4 MCM6 MCM7 MCM2 nucleus nucleus nucleus nucleus cytoplasm cytoplasm cytoplasm cytoplasm WHI5 bud WHI5 bud WHI5 bud WHI5 bud WHI5 mother WHI5 mother WHI5 mother WHI5 mother WHI5 MCM6 bud MCM6 bud MCM6 bud MCM6 bud MCM6 mother MCM6 mother MCM6 mother MCM6 mother MCM6

4. Expression intensity Distance between Distance to pattern cen. Distance to periphery Distance to cell centre Distance to bud neck Nucleus and cytoplasm (DNA binding and repair) Vacuole Nucleolus Nucleus Proteasome Kinetochore ER Translation Cytoplasm and nucleus (biosynthesis?) Golgi Mitochondrion Vacuole Vacuolar membrane Cytoplasm Cell periphery Cytoplasm Ambiguous, bud site Nucleus and cytoplasm (DNA binding and repair) ER Mitochondrion Bud neck Golgi, peroxisome ER