Microbial Flora on Local Currency: A Comparative Study
This experiment observed normal microflora found on money from local establishments using SEM. Results show significant diversity and amounts of microflora on various currency samples.
Microbial Flora on Local Currency: A Comparative Study
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Presentation Transcript
Matt Larson–Simon Missy Boevers Cory Haeffner Scott Suhr Dirty Money INTRODUCTION: The purpose of this experiment was to observe the normal micro flora found on money obtained from local establishments. • MATERIALS AND METHODS: • Materials • Money from five local banks and businesses • Trypticase Soy Broth • Hytachi 2406 Scanning Electron Microscope, dehydration solution, sputter coater • Methods • A subsample of bills were immersed in 25 ml of Tryp Soy Broth, and incubated at room temperature for four days. • Samples were removed from broth and fumigated in 37% formaldehyde fumes for 48 hours. • Samples were dehydrated with a 5% to 100 % ethanol series finished with HMDS. Samples were exposed for five minutes solution except for 100% and HMDS (ten minutes). • Samples were air dried on microscope stubs and sputter-coated with gold. • Images were taken using a Hitachi 2406 SEM in the SE mode. Results: First National Bank, Waverly, 11k magnification Hy-Vee, 1k magnification Hy-Vee, 10k magnification Pizza Ranch, Waverly, 10k magnification First National Bank, Waverly, 1k magnification Covenant Hospital, 10k magnification • Conclusion • Money does have a significant amount and diversity of micro flora