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Illienko Vladimir Department of Ecology and Sustainable Development

National University of Life and Environmental Sciences Department of Radiobiology and Radioecology. Comparative characteristics of the structure and biodiversity of soil  microflora  in areas contaminated with radionuclides and SS NULES of Ukraine Educational-research farm " Vorzel ”.

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Illienko Vladimir Department of Ecology and Sustainable Development

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  1. National University of Life and Environmental Sciences Department of Radiobiology and Radioecology Comparative characteristics of the structure and biodiversity of soil microflora in areas contaminated with radionuclides and SS NULES of Ukraine Educational-research farm "Vorzel” Illienko Vladimir Department of Ecology and Sustainable Development Supervisors: Doctor of Biological Sciences, Prof. Gudkov I.M. Candidate of Agricultural Science, Assistant Prof. PalamarchukS.P.

  2. Actuality of the topic After the Chernobyl accident had accumulated much data on the effects of ionizing radiation on the fauna and flora, but least studied effects of the disaster for prokaryotes. Given the critical role of soil microorganisms in providing and maintaining soil fertility, the redistribution of nutrients and cleansing of pollutants such research is extremely important today.

  3. Microbial complex of sod-podzolic soils that formed under different levels of radionuclide contamination of territories Object of research Effect of radioactive components on the soil microflora Subject of research

  4. Purpose: To study the biodiversity and structure of soil microbial complex formed in the territories contaminated with radionuclides Tasks: 1) on the investigated areas to measured gamma survey and to make soil sampling; 2) to determine the intensity of surface contamination by137Cs and 90Sr; 3) to assess the biodiversity of soil microorganisms in the conditions of radionuclide contamination of Chernobyl fallout; 4) to weed the dominant morphotypes in pure culture for further species identification; 5) to analyze the impact of radionuclide contamination on soil microflora composition.

  5. Methods • Sampling was carried out by standard methods: • soil – method of “envelope”, in accordance with State standard of Ukraine Б В.2.1-8-2001 (GOST12071-2000) andState standard of Ukraine 4287:2004; • Before the measurements all samples were properly prepared. • Measurements: • gamma-ray background – dosimeter-roentgenometer "Terra”; • 137Cs content in the samples - gamma-spectrometric method on RUB-01-P6. • Classical methods of microbiological testing

  6. Description of places for sampling

  7. Location of sampling sites Exclusion Zone ERF “Vorzel”

  8. 137Cs and 90Sr contamination of investigated soils

  9. Structure and quantitative composition of functional groups of microorganisms

  10. Biodiversity indexes of soil microorganisms communities

  11. The total quantity of microorganisms for the investigated microcenosis, colony units

  12. Comparison of microcenosis ERF “Vorzel”

  13. Conclusions: 1. Variation of surface soil contamination by 137Cs on soil sampling areas ranging from 26,2 ± 1,5 kBq/m2 (meadowsERF "Vorzel") to 28 000 ± 25 kBq/m2 (v. Chystohalivka), contamination by 90Sr from 3,9 ± 0,02 kBq/m2 to 15000 ± 47 kBq/m2 respectively 2. Results of multysubstrat testing showed that the investigated areas are such functional groups of microorganisms: ammonificatjrs, oligonitrophiles, microorganisms that use organic and mineral forms of phosphorus

  14. 3. Analysis of microorganisms isolated from soil extracts showed that the biocoenosis, characterized by the lowest level of radionuclide contamination have the highest number of microorganisms. At the same time followed by the number of representatives of all microorganisms groups was the area of v. Kopachi and v. Chystohalivka, but its level of radionuclide contamination is the largest from analyzed soils 4. For the territory of ERF "Vorzel": the number and diversity of microorganisms is higher for meadow biocenosis and it equaled 75 colony units, represented by 14 morphotypes. For forest biocenosis, which has twice higher specific activity of soil was found 29 colony units represented by 10 morphotypes

  15. Thank you for attention! The research results reported Паренюк О.Ю., Іллєнко В.В. Оцінка структури ґрунтової мікрофлори радіонуклідно забруднених територій. – Львів, 2011 р. Moscow Summer Ecology School (MOSES -2011): "Agro-Ecological Management and Engineering“. – 2011 Паренюк О.Ю., Іллєнко В.В. Аналіз функціональних груп грунтових мікроорганізмів з територій підданих радіонуклідному забрудненню. – Київ, НУБіП, 2011 р. I express my gratitude for the help: Director UIAR Prof. V.A. Kashparov,postgraduate student of radiobiology and radioecology department O.Y. Parenyuk

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