OBJ. 4 MAB PIPELINE ESTABLISHMENT & IMPLEMENTATION

1. OBJ. 4 MAB PIPELINEESTABLISHMENT & IMPLEMENTATION Year 3 Deliverables, Challenges,Year 4 Goals

2. Goal What • Integrate modern genomics tools with traditional breeding approaches, systematically (8-stage MAB Pipeline) Impact • Enrich breeding material for favorable geneticsto efficiently increase likelihood and accuracyof identifying top performers

3. Fruit color Year 3 Deliverables What Bitter pit susceptibility • Many Jewels available, some already in routine use “Fresh sensation”(crispness, tartness, juiciness) Fruit storability Ever-bearing Fruit size Self-fertility Fruit skin color Fruit size Freestone vs. clingstone Melting vs. Non-melting flesh

4. Year 3 Deliverables Red stele resistance Fruit color What • Many Jewels available, some already in routine use Sweetness & acidity Acidity Sunburn susceptibility To whom Leaf spot resistance • Demonstration breeding programs Impact • est. $60Ksavedfrom2012apple seedlingcullingalone! Maturity date

5. Challenges and Plan for tackling • More attention needed on translation to application (MAB Pipelining), less on QTL discovery • Such translation is: - idiosyncratic to each breeding program - new to breeders and students • Solution: MAB Pipelining TaskforceAttention to each crop, each program represented • Focus on: - marker conversion - allele effect calculations - allele distributions in germplasm - use in breeding

6. Planned Year 4 Deliverables What • More jewels!+ theskills&knowledgetodevelopandusetheseandmore Fruit size Firmness Capping ease To whom • Demonstration & Community Breeders Intended Impact • Success stories & experiences useful for encouraging and guiding MAB for all U.S. Rosaceae breeders Nectarine vs. peach

7. Planned Year 4 Deliverables • What • Breeder’s crossing and seedling selection decisions will be DNA-informed • To whom • Breeders • Intended Impact • More efficient development of new cultivars: • crosses yield more superior seedlings • early elimination of seedlings predicted to be genetically inferior

8. OBJ. 5 EXTENSION Year 3 Deliverables, Challenges, Year 4 Goals

9. Goals • Breeders: adoption of Marker-Assisted Breeding • Allied Scientists: utilization of newly generated science based knowledge, tools and databases • Industry: better understanding of the power of DNA-informed breeding leads to new ways to jointly accelerate commercialization and impact of new cultivars

10. Year 3 Deliverables What • RosBREED Participatory Workshop, 30 July 2012: • “Marker-Assisted Breeding in Action!” To whom • Community Breedersallied scientists Request from breeders: more information on QTL and validation studies, especially within their programs Impact • Comprehension by breeders of available knowledge, technologies and software for conducting routine MAB; application of MAB in their breeding programs

11. Year 3 Deliverables What 1. Plant & Animal Genome XX Conference, San Diego, CA, Jan 2012 - Presentations in Fruit & Nut Crops Workshop 2. 2012 FruitBreedomics First Annual Meeting, Prague, Czech Republic, Feb 2012 3. 2012 Biotechfruit 2012, Nelson, New Zealand, Mar 2012 4. 2012 American Society of Horticultural Science, Miaimi, FL, Aug 2012 5. 2012 Northwest Nursery Improvement Institute, Yakima, WA, Dec 2012 • Expansion of RosBREED network through presentations at meetings and informal gatherings – conducted To whom • Breeders, allied scientists, industry stakeholders Impact • Awareness and interest created at national and international meetings including “unveiling” of Jewels Fruit breeding working group sponsored RosBREED workshop at ASHS 2012

12. Year 3 Deliverables What • Project-wide content – developed, disseminated and undertaken Newsletter sent to 169 in 2012 (vs. 130 last year) To whom • Project participants & stakeholders Impact 94 recipients opened the Nov 2012 newsletter (vs. 79 last year) • Stakeholders gain knowledge of project activities and deliverables RosBREED website visited by 3,168 (vs. 1,353 in 2011) unique visitors, from 87 (vs. 42 in 2011) countries (52% returning & 48% new visitors!) “RosBREED Jewels are now featured in eXtension!!!”

13. Challenges and Plan for Tackling • Obtain participation of a high proportion of Rosaceae Community Breeders at the “MAB in Action” Workshop: held workshop in conjunction with ASHS • Learning from the experience: Hold Crop-Specific MAB in Action Workshops prior to or immediately after major fruit meetings; generate interest in Workshops through publicizing success stories • Peach Workshop: was held just prior to a South Carolina Peach Council Meeting • Apple workshop: immediately before PAG meeting • Strawberry workshop: immediately after NASGA meeting

14. Planned Year 4 Deliverables What • Conduct RosBREED Crop-Specific MAB Workshops • Peach - SC in Oct 2012; Apple - tomorrow in CA; Strawberry - January in OR • Visit breeding programson request to share RosBREED’s deliverables To whom • Demonstration and Community breeders Impact • Plan spring crosses using knowledge of DNA information; Comprehension by breeders of available knowledge, technologies and software for conducting routine MAB; Application of MAB in their breeding programs

15. Planned Year 4 Deliverables What • Continue to disseminate RosBREED deliverables and communicate with stakeholders through the use of published materials and participation in meetings To whom • Rosaceae breeders, allied scientists, industry Intended Impact • Breeders: breed as efficiently, accurately, and creativelyas possible • Allied scientists: connect better with breeders; use newly generated science-based knowledge, tools, databases • Industry: value & support DNA-informed breeding