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Study of miRNA Profiles in Adult and Pediatric Melanomas and Nevi Using FFPE Tissue Samples

This research investigates the miRNA expression profiles in formalin-fixed paraffin-embedded (FFPE) tissue samples of various melanoma types, categorized into adult and pediatric cohorts. Adult samples include primary melanomas (AM) and benign nevi (AN) from patients over 60 years old, while pediatric samples consist of young adult and pediatric melanomas (PM) and nevi (PN) from individuals under 30. Each cohort utilized 30-5μm cuts from areas containing at least 70% tumor content, with a focus on understanding neoplastic behaviors of melanocytic lesions of uncertain biology.

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Study of miRNA Profiles in Adult and Pediatric Melanomas and Nevi Using FFPE Tissue Samples

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  1. Study Schema Primary FFPE melanomas including melanocytic neoplasms of uncertain biological Benign nevi- homologous controls TLDA A 377 miRs TLDA B 289miRs 10 Adult melanomas “AM” 3 Adult Nevi“AN” 10+3 10+3 Cohort A > 60 yrs 30-5um cuts/FFPE 30-5um cuts from area containing at least 70% tumor /FFPE 10 Young Adult and Pediatric Melanomas “PM” 3 Young Adult and Pediatric Nevi“PN” 10+3 10+3 Cohort B <30 yrs 30-5um cuts from area containing at least 70% tumor /FFPE 30-5um cuts/FFPE

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