1 / 9

Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions

Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions. Víctor Rodríguez-Santiago EGEE 520. Background. Electrophoresis What? Movement of charged particles in aqueous solution under the influence of an external electric field. Uses?

jamesc
Télécharger la présentation

Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions Víctor Rodríguez-Santiago EGEE 520

  2. Background • Electrophoresis What? Movement of charged particles in aqueous solution under the influence of an external electric field. Uses? Protein separation, DNA migration studies, contaminant detection and separation. • Electroosmosis What? Movement of fluid under the influence of an external electric field. (Undesired in electrophoretic measurements)

  3. Background • Methods to prevent electroosmosis Polymeric coatings Buffer solutions Radial electric fields (Do not work for high temperatures) • Modeling to quantify electroosmosis • What causes electroosmosis? Electrical Double Layer

  4. Governing Equations • Using COMSOL™: Navier-Stokes mode Electrostatic mode Combined

  5. Solution Stagnant Layer Back flow Electroosmotic flow

  6. Validation • Experimental data was used to validate the model. With the modeling data, the experimental electrophoretic data can be corrected for electroosmosis. At pH 7: Experimental electrophoretic data: 1.76E-8 m2V-1s-1 Electroosmotic modeled data: 2.09E-9 m2V-1s-1 Corrected data: 1.55E-8 m2V-1s-1 The corrected value is well within the confidence value of the experimental data.

  7. Parametric Study

  8. Parametric Study

  9. Concluding Remarks • The COMSOL simulation gives reasonable values for the electroosmotic mobility. • When used to correct for the experimental data, the correction falls within the confidence values. • Either the electroosmotic effect is small or the simulation underestimates its value.

More Related