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This study presents the amplification of novel isoforms from four different brain regions, specifically the frontal cortex, cerebrum, cerebellum, and brainstem. We analyzed the amplification curves of specific splice isoforms in brain samples using advanced PCR techniques. For ZMYM6 and TRAP150 variants, RNA was amplified via SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR with ROX. CYFIP1 variants were quantified using TaqMan® assays with Gene Expression master mix. This work provides insights into the differential expression of gene isoforms across brain regions.
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CYFIP1-E16 : all but Frontal cortex amplified CYFIP1-Variant 1: all but Frontal cortex amplified ZMYM6-E2&5: all but Brain stem amplified ZMYM6-E4: all but Brain stem amplified ZMYM6-E2&4: all 4 samples amplified Supplementary Figure 3. TRAP150-E4: all 4 samples amplified
Supplementary Figure 3S.Amplification of novel isoforms from four different brain regions. PCR amplification curves from specific splice isoforms in four brain samples (frontal cortex, cerebrum, cerebellum, and brainstem) are illustrated.For ZMYM6 and TRAP150 variants, RNA was amplified using SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR w/ROX. For CYFIP1, TaqMan® assays were run using Gene Expression master mix. These real-time PCR reactions were run on an ABI 7000.
